TY - GEN
T1 - Nano-PCR
T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
AU - Lin, Yu Cheng
AU - Wu, Hua Lin
PY - 2007
Y1 - 2007
N2 - We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.
AB - We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.
UR - http://www.scopus.com/inward/record.url?scp=50049109462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049109462&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2007.4300150
DO - 10.1109/SENSOR.2007.4300150
M3 - Conference contribution
AN - SCOPUS:50049109462
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 391
EP - 394
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Y2 - 10 June 2007 through 14 June 2007
ER -