Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.

Original languageEnglish
Title of host publicationTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages391-394
Number of pages4
DOIs
Publication statusPublished - 2007 Dec 1
Event4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
Duration: 2007 Jun 102007 Jun 14

Publication series

NameTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
CountryFrance
CityLyon
Period07-06-1007-06-14

Fingerprint

Denaturation
Polymerase chain reaction
Melting point
Nanoparticles
Temperature
Colloids
DNA
Gold

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Lin, Y-C., & Wu, H-L. (2007). Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 391-394). [4300150] (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300150
Lin, Yu-Cheng ; Wu, Hua-Lin. / Nano-PCR : Breaking the bottom limit of the PCR denaturation temperature using nanogold. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. pp. 391-394 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).
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abstract = "We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.",
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Lin, Y-C & Wu, H-L 2007, Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold. in TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300150, TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 391-394, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, France, 07-06-10. https://doi.org/10.1109/SENSOR.2007.4300150

Nano-PCR : Breaking the bottom limit of the PCR denaturation temperature using nanogold. / Lin, Yu-Cheng; Wu, Hua-Lin.

TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 391-394 4300150 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lin Y-C, Wu H-L. Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 391-394. 4300150. (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300150