Nanofabricated SERS-active substrates for single-molecule to virus detection in vitro: A review

Shyh Chyang Luo, Kundan Sivashanmugan, Jiunn Der Liao, Chih Kai Yao, Han Chi Peng

Research output: Contribution to journalArticlepeer-review

248 Citations (Scopus)

Abstract

The surface-enhanced Raman scattering (SERS) method has great potential for the detection of Raman-active species, ranging from single molecules to biomolecules. In the last five years, various approaches have been developed to fabricate SERS-active substrates with high sensitivity using noble metal nanostructures via top-down, bottom-up, combination, or template-assisted routes. Nanostructured substrates with high average SERS enhancement factors (EFs) can now be easily produced, with the EF depending strongly on the size and shape of the nanostructures that give rise to the effect. For SERS substrates to be used as a platform for applications such as trace detection and bio-sensing, several issues, including sensitivity, intensity-concentration dependency, and selectivity, need to be addressed. Although several challenges remain before SERS-active substrates become consistent analytical tools, many successful examples have been demonstrated with promising results.

Original languageEnglish
Pages (from-to)232-240
Number of pages9
JournalBiosensors and Bioelectronics
Volume61
DOIs
Publication statusPublished - 2014 Nov 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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