Abstract
In this study, we prepared nanogels of a disulfide-cleavable polymer via polyionic complexation and genipin cross-linking and evaluated their reduction-triggered intracellular curcumin (Cur) delivery. These nanogels were stable at physiological conditions due to the formation of genipin cross-linking and helical PLL/PDC complexes and would swell/dissociate at acidic and reductive conditions due to the dissociation of PLL/PDC complexes and cleaving of disulfide bonds. The cellular uptake and intracellular release of Cur-loaded nanogels were demonstrated by tracking the fluorescent Cur and in vitro drug release studies, confirming the triggered release of Cur at acidic and reductive microenvironments in cells. The MTT, TUNEL staining, and Caspase-3 activity assays showed that the Cur-loaded nanogels exhibited higher cellular proliferation inhibition toward U-87 MG cells than free Cur, whereas the blank nanogels exhibited low cytoxicity. The results highlight the potential of functional nanogels prepared by polyionic complexation and cross-linking as a smart nanocarrier for drug delivery.
| Original language | English |
|---|---|
| Article number | 66 |
| Journal | Journal of Polymer Research |
| Volume | 24 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2017 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Polymers and Plastics
- Organic Chemistry
- Materials Chemistry
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Nanogels comprising reduction-cleavable polymers for glutathione-induced intracellular curcumin delivery. / Lee, Pei Yuan; Tuan-Mu, Ho Yi; Hsiao, Li Wen; Hu, Jin-Jia; Jan, Jeng-Shiung.
In: Journal of Polymer Research, Vol. 24, No. 5, 66, 01.04.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - Nanogels comprising reduction-cleavable polymers for glutathione-induced intracellular curcumin delivery
AU - Lee, Pei Yuan
AU - Tuan-Mu, Ho Yi
AU - Hsiao, Li Wen
AU - Hu, Jin-Jia
AU - Jan, Jeng-Shiung
PY - 2017/4/1
Y1 - 2017/4/1
N2 - In this study, we prepared nanogels of a disulfide-cleavable polymer via polyionic complexation and genipin cross-linking and evaluated their reduction-triggered intracellular curcumin (Cur) delivery. These nanogels were stable at physiological conditions due to the formation of genipin cross-linking and helical PLL/PDC complexes and would swell/dissociate at acidic and reductive conditions due to the dissociation of PLL/PDC complexes and cleaving of disulfide bonds. The cellular uptake and intracellular release of Cur-loaded nanogels were demonstrated by tracking the fluorescent Cur and in vitro drug release studies, confirming the triggered release of Cur at acidic and reductive microenvironments in cells. The MTT, TUNEL staining, and Caspase-3 activity assays showed that the Cur-loaded nanogels exhibited higher cellular proliferation inhibition toward U-87 MG cells than free Cur, whereas the blank nanogels exhibited low cytoxicity. The results highlight the potential of functional nanogels prepared by polyionic complexation and cross-linking as a smart nanocarrier for drug delivery.
AB - In this study, we prepared nanogels of a disulfide-cleavable polymer via polyionic complexation and genipin cross-linking and evaluated their reduction-triggered intracellular curcumin (Cur) delivery. These nanogels were stable at physiological conditions due to the formation of genipin cross-linking and helical PLL/PDC complexes and would swell/dissociate at acidic and reductive conditions due to the dissociation of PLL/PDC complexes and cleaving of disulfide bonds. The cellular uptake and intracellular release of Cur-loaded nanogels were demonstrated by tracking the fluorescent Cur and in vitro drug release studies, confirming the triggered release of Cur at acidic and reductive microenvironments in cells. The MTT, TUNEL staining, and Caspase-3 activity assays showed that the Cur-loaded nanogels exhibited higher cellular proliferation inhibition toward U-87 MG cells than free Cur, whereas the blank nanogels exhibited low cytoxicity. The results highlight the potential of functional nanogels prepared by polyionic complexation and cross-linking as a smart nanocarrier for drug delivery.
UR - http://www.scopus.com/inward/record.url?scp=85016974691&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016974691&partnerID=8YFLogxK
U2 - 10.1007/s10965-017-1207-6
DO - 10.1007/s10965-017-1207-6
M3 - Article
AN - SCOPUS:85016974691
VL - 24
JO - Journal of Polymer Research
JF - Journal of Polymer Research
SN - 1022-9760
IS - 5
M1 - 66
ER -