Nanogels comprising reduction-cleavable polymers for glutathione-induced intracellular curcumin delivery

Pei Yuan Lee, Ho Yi Tuan-Mu, Li Wen Hsiao, Jin-Jia Hu, Jeng-Shiung Jan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, we prepared nanogels of a disulfide-cleavable polymer via polyionic complexation and genipin cross-linking and evaluated their reduction-triggered intracellular curcumin (Cur) delivery. These nanogels were stable at physiological conditions due to the formation of genipin cross-linking and helical PLL/PDC complexes and would swell/dissociate at acidic and reductive conditions due to the dissociation of PLL/PDC complexes and cleaving of disulfide bonds. The cellular uptake and intracellular release of Cur-loaded nanogels were demonstrated by tracking the fluorescent Cur and in vitro drug release studies, confirming the triggered release of Cur at acidic and reductive microenvironments in cells. The MTT, TUNEL staining, and Caspase-3 activity assays showed that the Cur-loaded nanogels exhibited higher cellular proliferation inhibition toward U-87 MG cells than free Cur, whereas the blank nanogels exhibited low cytoxicity. The results highlight the potential of functional nanogels prepared by polyionic complexation and cross-linking as a smart nanocarrier for drug delivery.

Original languageEnglish
Article number66
JournalJournal of Polymer Research
Volume24
Issue number5
DOIs
Publication statusPublished - 2017 Apr 1

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Curcumin
Phase locked loops
Complexation
Glutathione
Polymers
Drug delivery
Assays
Disulfides
Caspase 3
NanoGel
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

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Nanogels comprising reduction-cleavable polymers for glutathione-induced intracellular curcumin delivery. / Lee, Pei Yuan; Tuan-Mu, Ho Yi; Hsiao, Li Wen; Hu, Jin-Jia; Jan, Jeng-Shiung.

In: Journal of Polymer Research, Vol. 24, No. 5, 66, 01.04.2017.

Research output: Contribution to journalArticle

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