TY - GEN
T1 - Nanoparticle-assisted high efficient polymerase chain reaction
AU - Lin, Yu Cheng
AU - Wu, Hua Lin
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2008
Y1 - 2008
N2 - This paper reports that the Au nanoparticles could dramatically enhance efficiency of polymer chain reaction (PCR) and shorten reaction time. The excellent energy transfer property of the nanoparticles should be the major factor in improving the PCR efficiency. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the PCR efficiency was increased. The results demonstrated that Au nanoparticles increase the sensitivity of PCR detection 5-10 fold in a slower PCR system (i.e. conventional PCR) and at least 104 fold in a quicker PCR system (i.e. real-time PCR), This innovation could improve the PCR efficiency using non-expensive polymerases, and general PCR reagent.
AB - This paper reports that the Au nanoparticles could dramatically enhance efficiency of polymer chain reaction (PCR) and shorten reaction time. The excellent energy transfer property of the nanoparticles should be the major factor in improving the PCR efficiency. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the PCR efficiency was increased. The results demonstrated that Au nanoparticles increase the sensitivity of PCR detection 5-10 fold in a slower PCR system (i.e. conventional PCR) and at least 104 fold in a quicker PCR system (i.e. real-time PCR), This innovation could improve the PCR efficiency using non-expensive polymerases, and general PCR reagent.
UR - http://www.scopus.com/inward/record.url?scp=69949144776&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69949144776&partnerID=8YFLogxK
U2 - 10.1115/MicroNano2008-70214
DO - 10.1115/MicroNano2008-70214
M3 - Conference contribution
AN - SCOPUS:69949144776
SN - 0791842940
SN - 9780791842942
T3 - 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
SP - 179
EP - 182
BT - 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
T2 - 2008 ASME 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
Y2 - 3 June 2008 through 5 June 2008
ER -