Nanoparticle-assisted high efficient polymerase chain reaction

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports that the Au nanoparticles could dramatically enhance efficiency of polymer chain reaction (PCR) and shorten reaction time. The excellent energy transfer property of the nanoparticles should be the major factor in improving the PCR efficiency. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the PCR efficiency was increased. The results demonstrated that Au nanoparticles increase the sensitivity of PCR detection 5-10 fold in a slower PCR system (i.e. conventional PCR) and at least 104 fold in a quicker PCR system (i.e. real-time PCR), This innovation could improve the PCR efficiency using non-expensive polymerases, and general PCR reagent.

Original languageEnglish
Title of host publication2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
Pages179-182
Number of pages4
DOIs
Publication statusPublished - 2008 Dec 1
Event2008 ASME 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008 - Kowloon, Hong Kong
Duration: 2008 Jun 32008 Jun 5

Publication series

Name2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008

Other

Other2008 ASME 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
CountryHong Kong
CityKowloon
Period08-06-0308-06-05

Fingerprint

Polymerase chain reaction
Nanoparticles
Polymers
Polymerase Chain Reaction
Energy Transfer
Computer Systems
Real time systems
Energy transfer
Innovation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Electrical and Electronic Engineering

Cite this

Lin, Y-C., & Wu, H-L. (2008). Nanoparticle-assisted high efficient polymerase chain reaction. In 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008 (pp. 179-182). (2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008). https://doi.org/10.1115/MicroNano2008-70214
Lin, Yu-Cheng ; Wu, Hua-Lin. / Nanoparticle-assisted high efficient polymerase chain reaction. 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008. 2008. pp. 179-182 (2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008).
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title = "Nanoparticle-assisted high efficient polymerase chain reaction",
abstract = "This paper reports that the Au nanoparticles could dramatically enhance efficiency of polymer chain reaction (PCR) and shorten reaction time. The excellent energy transfer property of the nanoparticles should be the major factor in improving the PCR efficiency. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the PCR efficiency was increased. The results demonstrated that Au nanoparticles increase the sensitivity of PCR detection 5-10 fold in a slower PCR system (i.e. conventional PCR) and at least 104 fold in a quicker PCR system (i.e. real-time PCR), This innovation could improve the PCR efficiency using non-expensive polymerases, and general PCR reagent.",
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Lin, Y-C & Wu, H-L 2008, Nanoparticle-assisted high efficient polymerase chain reaction. in 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008. 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008, pp. 179-182, 2008 ASME 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008, Kowloon, Hong Kong, 08-06-03. https://doi.org/10.1115/MicroNano2008-70214

Nanoparticle-assisted high efficient polymerase chain reaction. / Lin, Yu-Cheng; Wu, Hua-Lin.

2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008. 2008. p. 179-182 (2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Lin Y-C, Wu H-L. Nanoparticle-assisted high efficient polymerase chain reaction. In 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008. 2008. p. 179-182. (2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008). https://doi.org/10.1115/MicroNano2008-70214