Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip

Chun Ping Jen, Chun Sheng Fan, Yu-Cheng Lin, Yu-Hung Chen, Chia Wei Lee, Chen-Sheng Yeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Gold nanoparticles modified with 21-based thiolated-oligonucleotides have been utilized as novel gene delivery vehicles for electroporation on microchips in the present study. The electroporation chip, consisting of a defined cell culture cavity region with thin film electrodes made of titanium and gold, was fabricated on a glass slide using microfabrication technologies. The electric field for the Au-DNA conjugates attracting was generated to induce the electrophoresis of Au-DNA conjugates and enhance the transfection efficient on the electroporation microchip. Both the UV-vis spectra of the surface plasmon resonance and TEM micrographs indicated that the nanoparticles associated with DNA could be delivered into cells utilizing electroporation. This investigation had demonstrated the nanoparticle-based gene delivery system.

Original languageEnglish
Title of host publicationTRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1047-1050
Number of pages4
ISBN (Electronic)0780377311, 9780780377318
DOIs
Publication statusPublished - 2003 Jan 1
Event12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States
Duration: 2003 Jun 82003 Jun 12

Publication series

NameTRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
Volume2

Other

Other12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
CountryUnited States
CityBoston
Period03-06-0803-06-12

Fingerprint

DNA
Genes
Nanoparticles
Gold
Oligonucleotides
Microfabrication
Surface plasmon resonance
Electrophoresis
Cell culture
Titanium
Electric fields
Transmission electron microscopy
Glass
Thin films
Electrodes

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Jen, C. P., Fan, C. S., Lin, Y-C., Chen, Y-H., Lee, C. W., & Yeh, C-S. (2003). Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers (pp. 1047-1050). [1216948] (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers; Vol. 2). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SENSOR.2003.1216948
Jen, Chun Ping ; Fan, Chun Sheng ; Lin, Yu-Cheng ; Chen, Yu-Hung ; Lee, Chia Wei ; Yeh, Chen-Sheng. / Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip. TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 1047-1050 (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers).
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Jen, CP, Fan, CS, Lin, Y-C, Chen, Y-H, Lee, CW & Yeh, C-S 2003, Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip. in TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers., 1216948, TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers, vol. 2, Institute of Electrical and Electronics Engineers Inc., pp. 1047-1050, 12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers, Boston, United States, 03-06-08. https://doi.org/10.1109/SENSOR.2003.1216948

Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip. / Jen, Chun Ping; Fan, Chun Sheng; Lin, Yu-Cheng; Chen, Yu-Hung; Lee, Chia Wei; Yeh, Chen-Sheng.

TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Institute of Electrical and Electronics Engineers Inc., 2003. p. 1047-1050 1216948 (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - Gold nanoparticles modified with 21-based thiolated-oligonucleotides have been utilized as novel gene delivery vehicles for electroporation on microchips in the present study. The electroporation chip, consisting of a defined cell culture cavity region with thin film electrodes made of titanium and gold, was fabricated on a glass slide using microfabrication technologies. The electric field for the Au-DNA conjugates attracting was generated to induce the electrophoresis of Au-DNA conjugates and enhance the transfection efficient on the electroporation microchip. Both the UV-vis spectra of the surface plasmon resonance and TEM micrographs indicated that the nanoparticles associated with DNA could be delivered into cells utilizing electroporation. This investigation had demonstrated the nanoparticle-based gene delivery system.

AB - Gold nanoparticles modified with 21-based thiolated-oligonucleotides have been utilized as novel gene delivery vehicles for electroporation on microchips in the present study. The electroporation chip, consisting of a defined cell culture cavity region with thin film electrodes made of titanium and gold, was fabricated on a glass slide using microfabrication technologies. The electric field for the Au-DNA conjugates attracting was generated to induce the electrophoresis of Au-DNA conjugates and enhance the transfection efficient on the electroporation microchip. Both the UV-vis spectra of the surface plasmon resonance and TEM micrographs indicated that the nanoparticles associated with DNA could be delivered into cells utilizing electroporation. This investigation had demonstrated the nanoparticle-based gene delivery system.

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Jen CP, Fan CS, Lin Y-C, Chen Y-H, Lee CW, Yeh C-S. Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip. In TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers. Institute of Electrical and Electronics Engineers Inc. 2003. p. 1047-1050. 1216948. (TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers). https://doi.org/10.1109/SENSOR.2003.1216948