Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip

Chun Ping Jen, Chun Sheng Fan, Yu-Cheng Lin, Yu-Hung Chen, Chia Wei Lee, Chen-Sheng Yeh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Gold nanoparticles modified with 21-based thiolated-oligonucleotides have been utilized as novel gene delivery vehicles for electroporation on microchips in the present study. The electroporation chip, consisting of a defined cell culture cavity region with thin film electrodes made of titanium and gold, was fabricated on a glass slide using microfabrication technologies. The electric field for the Au-DNA conjugates attracting was generated to induce the electrophoresis of Au-DNA conjugates and enhance the transfection efficient on the electroporation microchip. Both the UV-vis spectra of the surface plasmon resonance and TEM micrographs indicated that the nanoparticles associated with DNA could be delivered into cells utilizing electroporation. This investigation had demonstrated the nanoparticle-based gene delivery system.

Original languageEnglish
Title of host publicationTRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1047-1050
Number of pages4
ISBN (Electronic)0780377311, 9780780377318
DOIs
Publication statusPublished - 2003 Jan 1
Event12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States
Duration: 2003 Jun 82003 Jun 12

Publication series

NameTRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
Volume2

Other

Other12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
CountryUnited States
CityBoston
Period03-06-0803-06-12

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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