TY - JOUR
T1 - Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli
AU - Tzeng, Shun Fen
AU - Tsai, May J.
AU - Hung, Shih Chieh
AU - Cheng, Henrich
N1 - Funding Information:
This study was supported, in part, by the National Health Research Institute (NHRI-GT-EX89B921C), Taipei Veterans General Hospital (VGH 91-390-10), and the National Science Council (NSC 91-2314-B-075-082).
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2004/8/26
Y1 - 2004/8/26
N2 - Size-sieved stem cells (SSCs) derived from human bone marrow have the ability to differentiate into bone, fat and cartilage. SSCs can differentiate into active neural cells after exposure to antioxidant agents. The aim of the present study is to understand if SSCs can be stimulated to differentiate into neurons in response to neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), pituitary adenylate cyclase-activating polypeptide (PACAP) and dibutyryl cAMP (dbcAMP). SSCs in a serum-free medium transform from a fibroblastic-like form to a multipolar morphology. Treatment of SSCs with GDNF, PACAP, and dbcAMP increased the production of neurofilament light protein (NF-L) and a cytoskeleton protein-α-tubulin. Examination of a vesicle protein-synapsin-1 or a neuronal progenitor marker-internexin in SSCs indicated that treatment with GDNF, PACAP, and dbcAMP further elongated cell processes and increased process branching. The findings indicate that neurotrophic signaling and cAMP-dependent signaling might promote the neuronal differentiation of SSCs.
AB - Size-sieved stem cells (SSCs) derived from human bone marrow have the ability to differentiate into bone, fat and cartilage. SSCs can differentiate into active neural cells after exposure to antioxidant agents. The aim of the present study is to understand if SSCs can be stimulated to differentiate into neurons in response to neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), pituitary adenylate cyclase-activating polypeptide (PACAP) and dibutyryl cAMP (dbcAMP). SSCs in a serum-free medium transform from a fibroblastic-like form to a multipolar morphology. Treatment of SSCs with GDNF, PACAP, and dbcAMP increased the production of neurofilament light protein (NF-L) and a cytoskeleton protein-α-tubulin. Examination of a vesicle protein-synapsin-1 or a neuronal progenitor marker-internexin in SSCs indicated that treatment with GDNF, PACAP, and dbcAMP further elongated cell processes and increased process branching. The findings indicate that neurotrophic signaling and cAMP-dependent signaling might promote the neuronal differentiation of SSCs.
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U2 - 10.1016/j.neulet.2004.05.117
DO - 10.1016/j.neulet.2004.05.117
M3 - Article
C2 - 15308290
AN - SCOPUS:4043171282
SN - 0304-3940
VL - 367
SP - 23
EP - 28
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -