Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli

Shun-Fen Tzeng, May J. Tsai, Shih Chieh Hung, Henrich Cheng

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Size-sieved stem cells (SSCs) derived from human bone marrow have the ability to differentiate into bone, fat and cartilage. SSCs can differentiate into active neural cells after exposure to antioxidant agents. The aim of the present study is to understand if SSCs can be stimulated to differentiate into neurons in response to neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), pituitary adenylate cyclase-activating polypeptide (PACAP) and dibutyryl cAMP (dbcAMP). SSCs in a serum-free medium transform from a fibroblastic-like form to a multipolar morphology. Treatment of SSCs with GDNF, PACAP, and dbcAMP increased the production of neurofilament light protein (NF-L) and a cytoskeleton protein-α-tubulin. Examination of a vesicle protein-synapsin-1 or a neuronal progenitor marker-internexin in SSCs indicated that treatment with GDNF, PACAP, and dbcAMP further elongated cell processes and increased process branching. The findings indicate that neurotrophic signaling and cAMP-dependent signaling might promote the neuronal differentiation of SSCs.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalNeuroscience Letters
Volume367
Issue number1
DOIs
Publication statusPublished - 2004 Aug 26

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Stem Cells
Pituitary Adenylate Cyclase-Activating Polypeptide
Glial Cell Line-Derived Neurotrophic Factor
Synapsins
Serum-Free Culture Media
Nerve Growth Factors
Tubulin
Cytoskeleton
Cartilage
Proteins
Antioxidants
Bone Marrow
Fats
Neurons
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Tzeng, Shun-Fen ; Tsai, May J. ; Hung, Shih Chieh ; Cheng, Henrich. / Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli. In: Neuroscience Letters. 2004 ; Vol. 367, No. 1. pp. 23-28.
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Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli. / Tzeng, Shun-Fen; Tsai, May J.; Hung, Shih Chieh; Cheng, Henrich.

In: Neuroscience Letters, Vol. 367, No. 1, 26.08.2004, p. 23-28.

Research output: Contribution to journalArticle

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