New insights into DNA-binding by type IIA topoisomerases

Chih Chiang Chang, Ying Ren Wang, Shin Fu Chen, Chyuan Chuan Wu, Nei Li Chan

Research output: Contribution to journalReview articlepeer-review

Abstract

Type IIA topoisomerases catalyze the passage of two DNA duplexes across each other to resolve the entanglements and coiling of cellular DNA. The ability of these enzymes to interact simultaneously but differentially with two DNA segments is central to their DNA-manipulating functions: one duplex DNA is bound and cleaved to produce a transient double-strand break through which another DNA segment can be transported. Recent structural analyses have revealed in atomic detail how type IIA enzymes contact DNA and how the enzyme-DNA interactions may be exploited by drugs to achieve therapeutic purposes. This review summarizes these new findings, with a special focus on the assembly and structural features of the enzymes' composite DNA-binding surfaces.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalCurrent Opinion in Structural Biology
Volume23
Issue number1
DOIs
Publication statusPublished - 2013 Feb

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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