New isoflavonoid glycosides and related constituents from astragali radix (astragalus membranaceus) and their inhibitory activity on nitric oxide production

Li Jie Zhang, Hui Kang Liu, Ping Chun Hsiao, Li Ming Yang Kuo, I. Jung Lee, Tian Shung Wu, Wen Fei Chiou, Yao Haur Kuo

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Twenty-four secondary metabolites, including 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (-)-methylinissolin 3-O-β-d-(6′-acetyl)-glucoside (1), (-)-methylinissolin 3-O-β-d-{6′-[(E)-but-2-enoyl]}-glucoside (2), and calycosin 7-O-β-d-(6″-acetyl)-glucoside (3) have been identified as new compounds on the basis of spectroscopic analysis; (-)-methylinissolin 3-O-β-d-glucoside (4) was isolated from the natural products for the first time. The nitric oxide (NO) production inhibitory activity of the major compounds has been assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. To identify A. membranaceus, a fingerprint method was developed by using a high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method. Furthermore, characteristic peaks for the 11 major compounds in the chromatogram were unambiguously confirmed.

Original languageEnglish
Pages (from-to)1131-1137
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number4
DOIs
Publication statusPublished - 2011 Feb 23

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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