Nifedipine, verapamil and diltiazem block shock-wave-induced rises in cytosolic calcium in MDCK cells

Chung Ren Jan, Wei Chuan Chen, Sheng-Nan Wu, Ching Jiunn Tseng

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Nifedipine and verapamil have been shown previously to protect against renal function alterations induced by shock wave lithotripsy (SWL) in humans and rats; however, the mechanism is unclear. This study was aimed to examine whether these drugs could protect cultured kidney cells following shock wave exposure (SWE). The effect of nifedipine, verapamil and diltiazem on Madin Darby canine kidney (MDCK) cells following SWE was examined by determining the release of glutamate oxalactate transferase (GOT) and lactate dehydrogenasse (LDH) in cell suspensions; and also cytosolic Ca 2+ concentration ([Ca 2+ ](i)). Immediately after SWE, there was a transient release of GOT and LDH (16% and 4 fold, respectively). In contrast, [Ca 2+ ](i) measured within 1-6 hr after SWE gradually increased by 15-156%. The Ca 2+ entry blockers (1 or 10 μM) failed to inhibit the enzyme release; however, they abolished the progressive rises in [Ca 2+ ](i). The Ca 2+ entry blockers may protect the cells from damage of SWE via maintaining a low resting [Ca 2+ ](i).

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalChinese Journal of Physiology
Volume41
Issue number4
Publication statusPublished - 1998 Jan 1

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

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