TY - JOUR
T1 - Nifedipine, verapamil and diltiazem block shock-wave-induced rises in cytosolic calcium in MDCK cells
AU - Jan, Chung Ren
AU - Chen, Wei Chuan
AU - Wu, Sheng Nan
AU - Tseng, Ching Jiunn
PY - 1998
Y1 - 1998
N2 - Nifedipine and verapamil have been shown previously to protect against renal function alterations induced by shock wave lithotripsy (SWL) in humans and rats; however, the mechanism is unclear. This study was aimed to examine whether these drugs could protect cultured kidney cells following shock wave exposure (SWE). The effect of nifedipine, verapamil and diltiazem on Madin Darby canine kidney (MDCK) cells following SWE was examined by determining the release of glutamate oxalactate transferase (GOT) and lactate dehydrogenasse (LDH) in cell suspensions; and also cytosolic Ca 2+ concentration ([Ca 2+ ](i)). Immediately after SWE, there was a transient release of GOT and LDH (16% and 4 fold, respectively). In contrast, [Ca 2+ ](i) measured within 1-6 hr after SWE gradually increased by 15-156%. The Ca 2+ entry blockers (1 or 10 μM) failed to inhibit the enzyme release; however, they abolished the progressive rises in [Ca 2+ ](i). The Ca 2+ entry blockers may protect the cells from damage of SWE via maintaining a low resting [Ca 2+ ](i).
AB - Nifedipine and verapamil have been shown previously to protect against renal function alterations induced by shock wave lithotripsy (SWL) in humans and rats; however, the mechanism is unclear. This study was aimed to examine whether these drugs could protect cultured kidney cells following shock wave exposure (SWE). The effect of nifedipine, verapamil and diltiazem on Madin Darby canine kidney (MDCK) cells following SWE was examined by determining the release of glutamate oxalactate transferase (GOT) and lactate dehydrogenasse (LDH) in cell suspensions; and also cytosolic Ca 2+ concentration ([Ca 2+ ](i)). Immediately after SWE, there was a transient release of GOT and LDH (16% and 4 fold, respectively). In contrast, [Ca 2+ ](i) measured within 1-6 hr after SWE gradually increased by 15-156%. The Ca 2+ entry blockers (1 or 10 μM) failed to inhibit the enzyme release; however, they abolished the progressive rises in [Ca 2+ ](i). The Ca 2+ entry blockers may protect the cells from damage of SWE via maintaining a low resting [Ca 2+ ](i).
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M3 - Article
C2 - 10099864
AN - SCOPUS:0032441979
SN - 0304-4920
VL - 41
SP - 181
EP - 188
JO - Chinese Journal of Physiology
JF - Chinese Journal of Physiology
IS - 4
ER -