NMDA receptor-independent epileptiform activity induced by magnesium-free solution in rat amygdala neurons is blocked by CNQX

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Abstract

The effect of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), a specific non-N-methyl-d-aspartate (non-NMDA) receptor antagonist, on NMDA-independent epileptiform activity induced by Mg2+-free medium was studied in rat basolateral amygdala (BLA) neurons using intracellular recording techniques. Twenty to 30 min after switching to Mg2+-free medium, spontaneous and evoked epileptiform activity were observed in 16 out of 18 amygdala slices. Superfusion of d-2-amino-5-phosphonovalerate (d-APV), a selective NMDA receptor antagonist, reduced the duration of epileptiform activity by an average of 83%. However, there was a residual depolarizing component which remained in the presence of d-APV. This d-APV-resistant component could be completely blocked by CNQX suggesting that it is mediated by non-NMDA receptors.

Original languageEnglish
Pages (from-to)53-55
Number of pages3
JournalNeuroscience Letters
Volume119
Issue number1
DOIs
Publication statusPublished - 1990 Oct 30

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6-Cyano-7-nitroquinoxaline-2,3-dione
2-Amino-5-phosphonovalerate
Amygdala
N-Methyl-D-Aspartate Receptors
Magnesium
Neurons
N-Methylaspartate
aspartic acid receptor

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "The effect of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), a specific non-N-methyl-d-aspartate (non-NMDA) receptor antagonist, on NMDA-independent epileptiform activity induced by Mg2+-free medium was studied in rat basolateral amygdala (BLA) neurons using intracellular recording techniques. Twenty to 30 min after switching to Mg2+-free medium, spontaneous and evoked epileptiform activity were observed in 16 out of 18 amygdala slices. Superfusion of d-2-amino-5-phosphonovalerate (d-APV), a selective NMDA receptor antagonist, reduced the duration of epileptiform activity by an average of 83{\%}. However, there was a residual depolarizing component which remained in the presence of d-APV. This d-APV-resistant component could be completely blocked by CNQX suggesting that it is mediated by non-NMDA receptors.",
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N2 - The effect of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), a specific non-N-methyl-d-aspartate (non-NMDA) receptor antagonist, on NMDA-independent epileptiform activity induced by Mg2+-free medium was studied in rat basolateral amygdala (BLA) neurons using intracellular recording techniques. Twenty to 30 min after switching to Mg2+-free medium, spontaneous and evoked epileptiform activity were observed in 16 out of 18 amygdala slices. Superfusion of d-2-amino-5-phosphonovalerate (d-APV), a selective NMDA receptor antagonist, reduced the duration of epileptiform activity by an average of 83%. However, there was a residual depolarizing component which remained in the presence of d-APV. This d-APV-resistant component could be completely blocked by CNQX suggesting that it is mediated by non-NMDA receptors.

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