TY - JOUR
T1 - Non-labeled virus detection using inverted triangular Au nano-cavities arrayed as SERS-active substrate
AU - Chang, Chia Wei
AU - Liao, Jiunn Der
AU - Shiau, Ai Li
AU - Yao, Chih Kai
N1 - Funding Information:
This work was supported by Top 100 University Advancement and Center for Micro/Nano Science and Technology of National Cheng Kung University , under grant numbers D98-2740 and D98-2700 .
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/8/10
Y1 - 2011/8/10
N2 - Virus detection is frequently based on antibody-based arrays and polymerase chain reactions. However, these methodologies have time-consuming incubation steps and obtaining clearly isolated target species is a complex process. In the present study, inverted triangular Au nano-cavities with various indentation depths and tip-to-tip displacements are well arrayed as a substrate for qualitative virus detection. The substrate is competent to entrap size-comparable target virus into nano-cavities, which as one, exerts its activity to form surface-enhanced Raman scattering particularly by the edges and cavities of the substrate. Through the induction of the electromagnetic effect by the substrate, the virus can be distinguished from the amino acids on its surface. The detectable concentration for encephalomyocarditis virus or adenovirus is 106 PFU/ml and that for influenza virus is 10 4 PFU/ml. The tailored substrate is enabled for fast-screening detection of target virus.
AB - Virus detection is frequently based on antibody-based arrays and polymerase chain reactions. However, these methodologies have time-consuming incubation steps and obtaining clearly isolated target species is a complex process. In the present study, inverted triangular Au nano-cavities with various indentation depths and tip-to-tip displacements are well arrayed as a substrate for qualitative virus detection. The substrate is competent to entrap size-comparable target virus into nano-cavities, which as one, exerts its activity to form surface-enhanced Raman scattering particularly by the edges and cavities of the substrate. Through the induction of the electromagnetic effect by the substrate, the virus can be distinguished from the amino acids on its surface. The detectable concentration for encephalomyocarditis virus or adenovirus is 106 PFU/ml and that for influenza virus is 10 4 PFU/ml. The tailored substrate is enabled for fast-screening detection of target virus.
UR - http://www.scopus.com/inward/record.url?scp=79957846028&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957846028&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2011.04.006
DO - 10.1016/j.snb.2011.04.006
M3 - Article
AN - SCOPUS:79957846028
SN - 0925-4005
VL - 156
SP - 471
EP - 478
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1
ER -