Noncoding effects of circular RNA CCDC66 promote colon cancer growth and metastasis

Kuei Yang Hsiao, Ya Chi Lin, Sachin Kumar Gupta, Ning Chang, Laising Yen, H. Sunny Sun, Shaw Jenq Tsai

Research output: Contribution to journalArticlepeer-review

504 Citations (Scopus)


Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circ-RNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-offunction studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis.

Original languageEnglish
Pages (from-to)2339-2350
Number of pages12
JournalCancer Research
Issue number9
Publication statusPublished - 2017 May 1

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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