TY - JOUR
T1 - Notch-1 signaling activation and progesterone receptor expression in ectopic lesions of women with endometriosis
AU - Brown, Dustin M.
AU - Lee, Hsiu Chi
AU - Liu, Shi
AU - Quick, Charles M.
AU - Fernandes, Lorenzo M.
AU - Simmen, Frank A.
AU - Tsai, Shaw Jenq
AU - Simmen, Rosalia C.M.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Context: Progesterone (P) resistance is a hallmark of endometriosis, but the underlying mechanism(s) for loss of P sensitivity leading to lesion establishment remains poorly understood. Objective: To evaluate the association between Notch-1 signaling activation and P resistance in the progression of endometriosis. Design: Case control study; archived formalin-fixed, paraffin-embedded tissues. Setting: University hospitals (United States, Taiwan). Patients: Women with endometriosis; human endometrial stromal cell line (HESC). Intervention: Eutopic endometria (EU) and ectopic lesions (ECs) were collected from surgically diagnosed patients. Archived tissue sections of EU and ECs were identified. HESCs were treated with N- [N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) and valproic acid (VPA) to, respectively, suppress and induce Notch-1 activation. Outcome Measures: Tissues were analyzed for Notch Intra-Cellular Domain 1 (NICD1) and progesterone receptor (PGR) protein expression by immunohistochemistry and for transcript levels of NICD1 target genes HES1, PGR, and PGR-B by quantitative reverse transcription polymerase chain reaction. DAPT- or VPA-treated HESCs with and without P cotreatment were evaluated for cell numbers and for PGR, HES1, and PGR target gene DKK1 transcript levels. Results: Nuclear-localized stromal NICD1 protein levels were inversely associated with those of total PGR in EU and ECs. Stromal ECs displayed higher HES1 and lower total PGR and PGR-B transcript levels than EU. In HESCs, DAPT reduction of NICD1 decreased cell numbers and increased PGR transcript and nuclear PGR protein levels and, with P cotreatment, maintained P sensitivity. Conversely, VPA induction of NICD1 decreased PGR transcript levels and, with P cotreatment, abrogated P-induced DKK1 and maintained HES1 transcript levels. Conclusions: Aberrant Notch-1 activation is associated with decreased PGR that contributes to P resistance in endometriosis.
AB - Context: Progesterone (P) resistance is a hallmark of endometriosis, but the underlying mechanism(s) for loss of P sensitivity leading to lesion establishment remains poorly understood. Objective: To evaluate the association between Notch-1 signaling activation and P resistance in the progression of endometriosis. Design: Case control study; archived formalin-fixed, paraffin-embedded tissues. Setting: University hospitals (United States, Taiwan). Patients: Women with endometriosis; human endometrial stromal cell line (HESC). Intervention: Eutopic endometria (EU) and ectopic lesions (ECs) were collected from surgically diagnosed patients. Archived tissue sections of EU and ECs were identified. HESCs were treated with N- [N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT) and valproic acid (VPA) to, respectively, suppress and induce Notch-1 activation. Outcome Measures: Tissues were analyzed for Notch Intra-Cellular Domain 1 (NICD1) and progesterone receptor (PGR) protein expression by immunohistochemistry and for transcript levels of NICD1 target genes HES1, PGR, and PGR-B by quantitative reverse transcription polymerase chain reaction. DAPT- or VPA-treated HESCs with and without P cotreatment were evaluated for cell numbers and for PGR, HES1, and PGR target gene DKK1 transcript levels. Results: Nuclear-localized stromal NICD1 protein levels were inversely associated with those of total PGR in EU and ECs. Stromal ECs displayed higher HES1 and lower total PGR and PGR-B transcript levels than EU. In HESCs, DAPT reduction of NICD1 decreased cell numbers and increased PGR transcript and nuclear PGR protein levels and, with P cotreatment, maintained P sensitivity. Conversely, VPA induction of NICD1 decreased PGR transcript levels and, with P cotreatment, abrogated P-induced DKK1 and maintained HES1 transcript levels. Conclusions: Aberrant Notch-1 activation is associated with decreased PGR that contributes to P resistance in endometriosis.
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U2 - 10.1210/JS.2018-00007
DO - 10.1210/JS.2018-00007
M3 - Article
SN - 2472-1972
VL - 2
SP - 765
EP - 778
JO - Journal of the Endocrine Society
JF - Journal of the Endocrine Society
IS - 7
ER -
