Novel Cellulase Screening and Optimal Production from the Wood Decaying Xylariaceae: Daldinia Species

The highest cellulases production from Daldinia caldariorum 263 (D-263) was found among Daldinia eschscholzii and Daldinia childiae. Three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105 kDa, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. From the N-terminal sequencing, the major cellulase CelA belonging to glycosyl hydrolase family 5 was determined. By following an orthogonal experiment design (L9), factors affecting the cultivation of D. caldariorum 263 are ranked as medium composition > temperature > pH ≥ FP (%). The optimum cultivation conditions for obtaining the best FPase (600 mU/ml) at 72 h are 150 rpm, 35 °C, pH 7, 0.2% soy peptone and 0.5% α-cellulose in minimal requirement medium. In comparison with Trichoderma reesei (ATCC26921) secreting 1,135 mU/ml of FPase after 6 days cultivation at pH 5, D. caldariorum 263 grew faster at 35 °C and produced the maximum FPase within 3 days at pH 7.