Novel Cellulase Screening and Optimal Production from the Wood Decaying Xylariaceae

Daldinia Species

I. Son Ng, Po Ting Chen, Yu Ming Ju, Shau Wei Tsai

Research output: Contribution to journalArticle

Abstract

The highest cellulases production from Daldinia caldariorum 263 (D-263) was found among Daldinia eschscholzii and Daldinia childiae. Three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105 kDa, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. From the N-terminal sequencing, the major cellulase CelA belonging to glycosyl hydrolase family 5 was determined. By following an orthogonal experiment design (L9), factors affecting the cultivation of D. caldariorum 263 are ranked as medium composition > temperature > pH ≥ FP (%). The optimum cultivation conditions for obtaining the best FPase (600 mU/ml) at 72 h are 150 rpm, 35 °C, pH 7, 0.2% soy peptone and 0.5% α-cellulose in minimal requirement medium. In comparison with Trichoderma reesei (ATCC26921) secreting 1,135 mU/ml of FPase after 6 days cultivation at pH 5, D. caldariorum 263 grew faster at 35 °C and produced the maximum FPase within 3 days at pH 7.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalApplied Biochemistry and Biotechnology
DOIs
Publication statusAccepted/In press - 2010

Fingerprint

Cellulases
Cellulase
Wood
Screening
Glucosidases
Peptones
Trichoderma
Hydrolases
Cellulose
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Molecular Weight
Sodium dodecyl sulfate
Polyacrylates
Electrophoresis
Temperature
Gels
Molecular weight
Chemical analysis
Experiments

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

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abstract = "The highest cellulases production from Daldinia caldariorum 263 (D-263) was found among Daldinia eschscholzii and Daldinia childiae. Three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105 kDa, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. From the N-terminal sequencing, the major cellulase CelA belonging to glycosyl hydrolase family 5 was determined. By following an orthogonal experiment design (L9), factors affecting the cultivation of D. caldariorum 263 are ranked as medium composition > temperature > pH ≥ FP ({\%}). The optimum cultivation conditions for obtaining the best FPase (600 mU/ml) at 72 h are 150 rpm, 35 °C, pH 7, 0.2{\%} soy peptone and 0.5{\%} α-cellulose in minimal requirement medium. In comparison with Trichoderma reesei (ATCC26921) secreting 1,135 mU/ml of FPase after 6 days cultivation at pH 5, D. caldariorum 263 grew faster at 35 °C and produced the maximum FPase within 3 days at pH 7.",
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Novel Cellulase Screening and Optimal Production from the Wood Decaying Xylariaceae : Daldinia Species. / Ng, I. Son; Chen, Po Ting; Ju, Yu Ming; Tsai, Shau Wei.

In: Applied Biochemistry and Biotechnology, 2010, p. 1-11.

Research output: Contribution to journalArticle

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