Novel essential gene involved in 16S rRNA processing in escherichia coli

Tatsuaki Kurata, Shinobu Nakanishi, Masayuki Hashimoto, Masato Taoka, Yukiko Yamazaki, Toshiaki Isobe, Jun Ichi Kato

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Biogenesis of ribosomes is a complex process mediated by many factors. While its transcription proceeds, ribosomal RNA (rRNA) folds itself into a characteristic three-dimensional structure through interaction with ribosomal proteins, during which its ends are processed. Here, we show that the essential protein YqgF, a RuvC family protein with an RNase-H-like motif, is involved in the processing of pre-16S rRNA during ribosome maturation. Indeed, pre-16S rRNA accumulated in cells of a temperature-sensitive yqgF mutant (yqgFts) cultured at a non-permissive temperature. In addition, purified YqgF was shown to process the 5′ end of pre-16S rRNA within 70S ribosomes in vitro. Mass spectrometry analysis of the total proteins in the yqgFts mutant cells showed that the expression of genes containing multiple Shine-Dalgarno-like sequences was observed to be lower than in wild type. These results are interpreted to indicate that YqgF is involved in a novel enzymic activity necessary for the processing of pre-16S rRNA, thereby affecting elongation of translation.

Original languageEnglish
Pages (from-to)955-965
Number of pages11
JournalJournal of Molecular Biology
Volume427
Issue number4
DOIs
Publication statusPublished - 2015 Feb 27

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16S Ribosomal RNA
Essential Genes
Ribosomes
Escherichia coli
Ribonuclease H
Proteins
Temperature
Ribosomal Proteins
Mass Spectrometry
Gene Expression

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Kurata, T., Nakanishi, S., Hashimoto, M., Taoka, M., Yamazaki, Y., Isobe, T., & Kato, J. I. (2015). Novel essential gene involved in 16S rRNA processing in escherichia coli. Journal of Molecular Biology, 427(4), 955-965. https://doi.org/10.1016/j.jmb.2014.12.013
Kurata, Tatsuaki ; Nakanishi, Shinobu ; Hashimoto, Masayuki ; Taoka, Masato ; Yamazaki, Yukiko ; Isobe, Toshiaki ; Kato, Jun Ichi. / Novel essential gene involved in 16S rRNA processing in escherichia coli. In: Journal of Molecular Biology. 2015 ; Vol. 427, No. 4. pp. 955-965.
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Kurata, T, Nakanishi, S, Hashimoto, M, Taoka, M, Yamazaki, Y, Isobe, T & Kato, JI 2015, 'Novel essential gene involved in 16S rRNA processing in escherichia coli', Journal of Molecular Biology, vol. 427, no. 4, pp. 955-965. https://doi.org/10.1016/j.jmb.2014.12.013

Novel essential gene involved in 16S rRNA processing in escherichia coli. / Kurata, Tatsuaki; Nakanishi, Shinobu; Hashimoto, Masayuki; Taoka, Masato; Yamazaki, Yukiko; Isobe, Toshiaki; Kato, Jun Ichi.

In: Journal of Molecular Biology, Vol. 427, No. 4, 27.02.2015, p. 955-965.

Research output: Contribution to journalArticle

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