Novel flow cytometrer utilizing wavelength-resovled detection under a diascopic illumination configuration

Shi Wei Lin, Chih-Han Chang, Chia Yen Lee, Lung-Ming Fu, Che Hsin Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper presents a novel yet high performance diascopic illumination configuration for simultaneously detecting cells of different sizes and different fluorescence labeling in a microfluidic chip. An objective-type dark-field condenser equipped with a low-cost tungsten bulb light source with continuous wavelengths (400 to 900 nm) and an UV-Vis-NIR spectrometer is used for detecting the multispectral signals from various particles and cells. With this approach, continuous cell counting and spectra analyzing are realized in a single channel without using any spatial filter and delicate optical detectors. Results show that the developed system is capable of identifying different labeled particle and cell samples by extracting the information side-scatter, absorption, and fluorescence from the information-rich spectra. This proposed system provides an efficient multi-color detection for identification and classification on a micro-flow cytometer chip.

Original languageEnglish
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages1987-1990
Number of pages4
DOIs
Publication statusPublished - 2010 Dec 1
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: 2010 Nov 12010 Nov 4

Publication series

NameProceedings of IEEE Sensors

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period10-11-0110-11-04

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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