TY - GEN
T1 - Novel flow cytometrer utilizing wavelength-resovled detection under a diascopic illumination configuration
AU - Lin, Shi Wei
AU - Chang, Chih Han
AU - Lee, Chia Yen
AU - Fu, Lung Ming
AU - Lin, Che Hsin
PY - 2010
Y1 - 2010
N2 - This paper presents a novel yet high performance diascopic illumination configuration for simultaneously detecting cells of different sizes and different fluorescence labeling in a microfluidic chip. An objective-type dark-field condenser equipped with a low-cost tungsten bulb light source with continuous wavelengths (400 to 900 nm) and an UV-Vis-NIR spectrometer is used for detecting the multispectral signals from various particles and cells. With this approach, continuous cell counting and spectra analyzing are realized in a single channel without using any spatial filter and delicate optical detectors. Results show that the developed system is capable of identifying different labeled particle and cell samples by extracting the information side-scatter, absorption, and fluorescence from the information-rich spectra. This proposed system provides an efficient multi-color detection for identification and classification on a micro-flow cytometer chip.
AB - This paper presents a novel yet high performance diascopic illumination configuration for simultaneously detecting cells of different sizes and different fluorescence labeling in a microfluidic chip. An objective-type dark-field condenser equipped with a low-cost tungsten bulb light source with continuous wavelengths (400 to 900 nm) and an UV-Vis-NIR spectrometer is used for detecting the multispectral signals from various particles and cells. With this approach, continuous cell counting and spectra analyzing are realized in a single channel without using any spatial filter and delicate optical detectors. Results show that the developed system is capable of identifying different labeled particle and cell samples by extracting the information side-scatter, absorption, and fluorescence from the information-rich spectra. This proposed system provides an efficient multi-color detection for identification and classification on a micro-flow cytometer chip.
UR - http://www.scopus.com/inward/record.url?scp=79951922218&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951922218&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2010.5689918
DO - 10.1109/ICSENS.2010.5689918
M3 - Conference contribution
AN - SCOPUS:79951922218
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 1987
EP - 1990
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -