TY - JOUR
T1 - Novel role of mfd
T2 - Effects on stationary-phase mutagenesis in Bacillus subtilis
AU - Ross, Christian
AU - Pybus, Christine
AU - Pedraza-Reyes, Mario
AU - Sung, Huang Mo
AU - Yasbin, Ronald E.
AU - Robleto, Eduardo
PY - 2006/11
Y1 - 2006/11
N2 - Previously, using a chromosomal reversion assay system, we established that an adaptive mutagenic process occurs in nongrowing Bacillus subtilis cells under stress, and we demonstrated that multiple mechanisms are involved in generating these mutations (41, 43). In an attempt to delineate how these mutations are generated, we began an investigation into whether or not transcription and transcription-associated proteins influence adaptive mutagenesis. In B. subtilis, the Mfd protein (transcription repair coupling factor) facilitates removal of RNA polymerase stalled at transcriptional blockages and recruitment of repair proteins to DNA lesions on the transcribed strand. Here we demonstrate that the loss of Mfd has a depressive effect on stationary-phase mutagenesis. An association between Mfd mutagenesis and aspects of transcription is discussed.
AB - Previously, using a chromosomal reversion assay system, we established that an adaptive mutagenic process occurs in nongrowing Bacillus subtilis cells under stress, and we demonstrated that multiple mechanisms are involved in generating these mutations (41, 43). In an attempt to delineate how these mutations are generated, we began an investigation into whether or not transcription and transcription-associated proteins influence adaptive mutagenesis. In B. subtilis, the Mfd protein (transcription repair coupling factor) facilitates removal of RNA polymerase stalled at transcriptional blockages and recruitment of repair proteins to DNA lesions on the transcribed strand. Here we demonstrate that the loss of Mfd has a depressive effect on stationary-phase mutagenesis. An association between Mfd mutagenesis and aspects of transcription is discussed.
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U2 - 10.1128/JB.00980-06
DO - 10.1128/JB.00980-06
M3 - Article
C2 - 16950921
AN - SCOPUS:33750461659
SN - 0021-9193
VL - 188
SP - 7512
EP - 7520
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 21
ER -