Novel role of mfd: Effects on stationary-phase mutagenesis in Bacillus subtilis

Christian Ross, Christine Pybus, Mario Pedraza-Reyes, Huang Mo Sung, Ronald E. Yasbin, Eduardo Robleto

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Previously, using a chromosomal reversion assay system, we established that an adaptive mutagenic process occurs in nongrowing Bacillus subtilis cells under stress, and we demonstrated that multiple mechanisms are involved in generating these mutations (41, 43). In an attempt to delineate how these mutations are generated, we began an investigation into whether or not transcription and transcription-associated proteins influence adaptive mutagenesis. In B. subtilis, the Mfd protein (transcription repair coupling factor) facilitates removal of RNA polymerase stalled at transcriptional blockages and recruitment of repair proteins to DNA lesions on the transcribed strand. Here we demonstrate that the loss of Mfd has a depressive effect on stationary-phase mutagenesis. An association between Mfd mutagenesis and aspects of transcription is discussed.

Original languageEnglish
Pages (from-to)7512-7520
Number of pages9
JournalJournal of Bacteriology
Volume188
Issue number21
DOIs
Publication statusPublished - 2006 Nov

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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