Nuclei location enhancement based on improved efficient ellipse hough transform for third harmonic generation microscopy imaging

Yi Hsuan Chou, Shi Yu Hung, Gwo Giun Chris Lee, Wei Hung Weng, Yi Hua Liao, Chi Kung Sun, Han Ting Shih

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Instead of conventional manual cell segmentation, this paper introduces a computer-Aided diagnosis protocol based on non-invasive in vivo virtual biopsy, third harmonic generation (THG) microscopy, which provides consistency and prompt suggestions for immediate clinical decisions without pain to patients. Specifically, we present a nuclei location re-inspection mechanism using enhanced efficient ellipse Hough transform with labeling and weighting information building upon previous watershed-based nuclei segmentation. This transform approach cautiously identifies nuclei location by considering not only staining intensity, but also examine shape feature to enhance the accuracy of nuclei recognition. The current approach has been implemented and tested on about 400 THG images from 25 healthy volunteers, and the receiver operating characteristic (ROC) curve has demonstrated the better performance of decreased false positive rate and increased true positive rate as compared to other nuclei location detection algorithms.

Original languageEnglish
Title of host publication2015 IEEE China Summit and International Conference on Signal and Information Processing, ChinaSIP 2015 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages807-811
Number of pages5
ISBN (Electronic)9781479919482
DOIs
Publication statusPublished - 2015 Aug 31
EventIEEE China Summit and International Conference on Signal and Information Processing, ChinaSIP 2015 - Chengdu, China
Duration: 2015 Jul 122015 Jul 15

Publication series

Name2015 IEEE China Summit and International Conference on Signal and Information Processing, ChinaSIP 2015 - Proceedings

Other

OtherIEEE China Summit and International Conference on Signal and Information Processing, ChinaSIP 2015
Country/TerritoryChina
CityChengdu
Period15-07-1215-07-15

All Science Journal Classification (ASJC) codes

  • Information Systems
  • Signal Processing

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