Numerical and experimental investigation of three-dimensional hydrodynamic focusing in Polydimethylsiloxane (PDMS) microchannels

Chih Chang Chang, Zhi Xiong Huang, Ruey Jen Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we have designed and fabricated a threedimensional hydrodynamic focusing microfluidic device. The device comprises two-layer PDMS microchannels structure. Sample flow stream was firstly vertically constrained into a narrow core-region, and then horizontally focused into one small window from cross-section perspective, which is useful for cell/particle counting. We have show the numerical and experimental images of the focused stream shape from cross-section perspective, experimental ones were captured using confocal fluorescence microscope. We have also investigated the effect of channel aspect ratio on vertical focusing effect using CFD simulations. The results show that the sample flow can be focused successfully in lower aspect ratio (~0.5) of main channel in our design. Furthermore, the effect of Reynolds number on the vertical focusing effect was also investigated. The numerical results show that the rectangular-like shape of the focused stream from cross-section perspective was deformed as Reynolds number is high due to stronger secondary flows was produced in vertical focusing element. This phenomenon was also demonstrated experimentally. In other words, the chip only works well at low Reynolds number (< 5). The device can be integrated into on-chip flow cytometry.

Original languageEnglish
Title of host publication2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
Pages324-327
Number of pages4
Publication statusPublished - 2007 Aug 23
Event2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007 - Santa Clara, CA, United States
Duration: 2007 May 202007 May 24

Publication series

Name2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
Volume3

Other

Other2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007
CountryUnited States
CitySanta Clara, CA
Period07-05-2007-05-24

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

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