Observation of peptide differences between cancer and control in gastric juice

Wei Chao Chang, Ping I. Hsu, Yuan Yan Chen, Michael Hsiao, Pei Jung Lu, Chung Hsuan Chen

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Biomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI-TOF-MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were dear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides; for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α-1-antitrypsin fragment, have been identified by mass spectrometric analysis and immuno-deplete assay with antibodies.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalProteomics - Clinical Applications
Volume2
Issue number1
DOIs
Publication statusPublished - 2008 Jan

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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