Observation of peptide differences between cancer and control in gastric juice

Wei Chao Chang, Ping I. Hsu, Yuan Yan Chen, Michael Hsiao, Pei-Jung Lu, Chung Hsuan Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Biomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI-TOF-MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were dear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides; for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α-1-antitrypsin fragment, have been identified by mass spectrometric analysis and immuno-deplete assay with antibodies.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalProteomics - Clinical Applications
Volume2
Issue number1
DOIs
Publication statusPublished - 2008 Jan 1

Fingerprint

Gastric Juice
Biomarkers
Observation
Peptides
Pepsinogen A
Leucine Zippers
Neoplasms
Duodenal Neoplasms
Stomach Diseases
Albumins
Assays
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteins
Stomach Ulcer
Duodenal Ulcer
Stomach Neoplasms
Blood Proteins
Antibodies
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Chang, Wei Chao ; Hsu, Ping I. ; Chen, Yuan Yan ; Hsiao, Michael ; Lu, Pei-Jung ; Chen, Chung Hsuan. / Observation of peptide differences between cancer and control in gastric juice. In: Proteomics - Clinical Applications. 2008 ; Vol. 2, No. 1. pp. 55-62.
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Observation of peptide differences between cancer and control in gastric juice. / Chang, Wei Chao; Hsu, Ping I.; Chen, Yuan Yan; Hsiao, Michael; Lu, Pei-Jung; Chen, Chung Hsuan.

In: Proteomics - Clinical Applications, Vol. 2, No. 1, 01.01.2008, p. 55-62.

Research output: Contribution to journalArticle

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