Abstract
Biomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI-TOF-MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were dear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides; for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α-1-antitrypsin fragment, have been identified by mass spectrometric analysis and immuno-deplete assay with antibodies.
| Original language | English |
|---|---|
| Pages (from-to) | 55-62 |
| Number of pages | 8 |
| Journal | Proteomics - Clinical Applications |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Clinical Biochemistry
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Observation of peptide differences between cancer and control in gastric juice. / Chang, Wei Chao; Hsu, Ping I.; Chen, Yuan Yan; Hsiao, Michael; Lu, Pei-Jung; Chen, Chung Hsuan.
In: Proteomics - Clinical Applications, Vol. 2, No. 1, 01.01.2008, p. 55-62.Research output: Contribution to journal › Article
TY - JOUR
T1 - Observation of peptide differences between cancer and control in gastric juice
AU - Chang, Wei Chao
AU - Hsu, Ping I.
AU - Chen, Yuan Yan
AU - Hsiao, Michael
AU - Lu, Pei-Jung
AU - Chen, Chung Hsuan
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Biomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI-TOF-MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were dear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides; for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α-1-antitrypsin fragment, have been identified by mass spectrometric analysis and immuno-deplete assay with antibodies.
AB - Biomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI-TOF-MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were dear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides; for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α-1-antitrypsin fragment, have been identified by mass spectrometric analysis and immuno-deplete assay with antibodies.
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UR - http://www.scopus.com/inward/citedby.url?scp=38949202419&partnerID=8YFLogxK
U2 - 10.1002/prca.200780066
DO - 10.1002/prca.200780066
M3 - Article
VL - 2
SP - 55
EP - 62
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
SN - 1862-8346
IS - 1
ER -