Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates

Ming Chi Li, Tsung C. Chang, Hung Mo Chen, Chi Jung Wu, Shu Li Su, Susan S.J. Lee, Po Lin Chen, Nan Yao Lee, Ching Chi Lee, Chia Wen Li, Ling Shan Syue, Wen Chien Ko

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

Original languageEnglish
Article number51
JournalFrontiers in Microbiology
Volume9
Issue numberJAN
DOIs
Publication statusPublished - 2018 Jan 26

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Oligonucleotide Array Sequence Analysis
Candida
Mass Spectrometry
Lasers
Yeasts
rRNA Genes
Databases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

@article{33c59005868c436a965cb8a5ed442cf9,
title = "Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates",
abstract = "We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6{\%} (508) and 96.9{\%} (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100{\%}, 98.4{\%}; C. glabrata 100{\%}, 100{\%}; C. parapsilosis 100{\%}, 93.3{\%}; C. tropicalis 100{\%}, 97.3{\%}), but lower correct identification rates for other Candida species (91.7 and 87.5{\%}, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.",
author = "Li, {Ming Chi} and Chang, {Tsung C.} and Chen, {Hung Mo} and Wu, {Chi Jung} and Su, {Shu Li} and Lee, {Susan S.J.} and Chen, {Po Lin} and Lee, {Nan Yao} and Lee, {Ching Chi} and Li, {Chia Wen} and Syue, {Ling Shan} and Ko, {Wen Chien}",
year = "2018",
month = "1",
day = "26",
doi = "10.3389/fmicb.2018.00051",
language = "English",
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Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates. / Li, Ming Chi; Chang, Tsung C.; Chen, Hung Mo; Wu, Chi Jung; Su, Shu Li; Lee, Susan S.J.; Chen, Po Lin; Lee, Nan Yao; Lee, Ching Chi; Li, Chia Wen; Syue, Ling Shan; Ko, Wen Chien.

In: Frontiers in Microbiology, Vol. 9, No. JAN, 51, 26.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates

AU - Li, Ming Chi

AU - Chang, Tsung C.

AU - Chen, Hung Mo

AU - Wu, Chi Jung

AU - Su, Shu Li

AU - Lee, Susan S.J.

AU - Chen, Po Lin

AU - Lee, Nan Yao

AU - Lee, Ching Chi

AU - Li, Chia Wen

AU - Syue, Ling Shan

AU - Ko, Wen Chien

PY - 2018/1/26

Y1 - 2018/1/26

N2 - We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

AB - We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

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JF - Frontiers in Microbiology

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