Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates

Ming Chi Li, Tsung C. Chang, Hung Mo Chen, Chi Jung Wu, Shu Li Su, Susan S.J. Lee, Po Lin Chen, Nan Yao Lee, Ching Chi Lee, Chia Wen Li, Ling Shan Syue, Wen Chien Ko

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

Original languageEnglish
Article number51
JournalFrontiers in Microbiology
Volume9
Issue numberJAN
DOIs
Publication statusPublished - 2018 Jan 26

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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