Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates

Ming-Chi Li, Tsung C. Chang, Hung Mo Chen, Chi Jung Wu, Shu Li Su, Susan S.J. Lee, Po-Lin Chen, Nan-Yao Lee, Ching Chi Lee, Chia-Wen Li, Ling-Shan Syue, Wen-Chien Ko

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

Original languageEnglish
Article number51
JournalFrontiers in Microbiology
Volume9
Issue numberJAN
DOIs
Publication statusPublished - 2018 Jan 26

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Oligonucleotide Array Sequence Analysis
Candida
Mass Spectrometry
Lasers
Yeasts
rRNA Genes
Databases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

@article{33c59005868c436a965cb8a5ed442cf9,
title = "Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates",
abstract = "We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6{\%} (508) and 96.9{\%} (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100{\%}, 98.4{\%}; C. glabrata 100{\%}, 100{\%}; C. parapsilosis 100{\%}, 93.3{\%}; C. tropicalis 100{\%}, 97.3{\%}), but lower correct identification rates for other Candida species (91.7 and 87.5{\%}, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.",
author = "Ming-Chi Li and Chang, {Tsung C.} and Chen, {Hung Mo} and Wu, {Chi Jung} and Su, {Shu Li} and Lee, {Susan S.J.} and Po-Lin Chen and Nan-Yao Lee and Lee, {Ching Chi} and Chia-Wen Li and Ling-Shan Syue and Wen-Chien Ko",
year = "2018",
month = "1",
day = "26",
doi = "10.3389/fmicb.2018.00051",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
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Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates. / Li, Ming-Chi; Chang, Tsung C.; Chen, Hung Mo; Wu, Chi Jung; Su, Shu Li; Lee, Susan S.J.; Chen, Po-Lin; Lee, Nan-Yao; Lee, Ching Chi; Li, Chia-Wen; Syue, Ling-Shan; Ko, Wen-Chien.

In: Frontiers in Microbiology, Vol. 9, No. JAN, 51, 26.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oligonucleotide array and VITEK matrix-assisted laser desorption ionization-time of flight mass spectrometry in species identification of blood yeast isolates

AU - Li, Ming-Chi

AU - Chang, Tsung C.

AU - Chen, Hung Mo

AU - Wu, Chi Jung

AU - Su, Shu Li

AU - Lee, Susan S.J.

AU - Chen, Po-Lin

AU - Lee, Nan-Yao

AU - Lee, Ching Chi

AU - Li, Chia-Wen

AU - Syue, Ling-Shan

AU - Ko, Wen-Chien

PY - 2018/1/26

Y1 - 2018/1/26

N2 - We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

AB - We evaluated matrix-assisted laser desorption ionization time-of-flight mass spectrometry using VITEK MS (IVD database) and an oligonucleotide array based on the internal transcribed spacer-1 (ITS-1) and ITS-2 sequences of rRNA genes for the identification of Candida spp. from blood cultures. Five-hundred and twelve consecutive bloodstream yeast isolates were collected daily and initially identified by the phenotypic automated method (VITEK YBC or VITEK2 YST card). Inconsistent results were confirmed by D1-D2 region of 28S rRNA genes and ITSs. Excluding two unidentified yeast isolates, the oligonucleotide array and VITEK MS correctly identified 99.6% (508) and 96.9% (494) of 510 yeast isolates, respectively. The oligonucleotide array and VITEK MS demonstrated high correct identification rates for four major Candida species (C. albicans 100%, 98.4%; C. glabrata 100%, 100%; C. parapsilosis 100%, 93.3%; C. tropicalis 100%, 97.3%), but lower correct identification rates for other Candida species (91.7 and 87.5%, respectively). In conclusion, the identification performance of the oligonucleotide array is comparable to that of VITEK MS, and can serve as a supplemental tool for the identification of Candida species.

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DO - 10.3389/fmicb.2018.00051

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JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

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