Oligonucleotides-assembled au nanorod-assisted cancer photothermal ablation and combination chemotherapy with targeted dual-drug delivery of doxorubicin and cisplatin prodrug

Vijayakumar Shanmugam, Yi Hsin Chien, Yu Sheng Cheng, Tzu Yu Liu, Chih Chia Huang, Chia Hao Su, Yu Sen Chen, Umesh Kumar, Hua Fen Hsu, Chen Sheng Yeh

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

External stimuli responsive dual drugs carrier was synthesized with Au nanorods (NRs) as the platform. On Au NRs, single stranded DNAs were assembled using 5′ thiol end. Following this, complementary DNA (cDNA) strands were hybridized. This hybridized double stranded DNA facilitated doxorubicin (Dox) intercalation into the duplexes. The cDNA designed with the 5′ amine functional group assisted to tether platinum [Pt(IV)] prodrugs by establishing amide bond with the acid group at the axial ligand. The other axial acid group in Pt(IV) prodrugs was conjugated with the folic acid (FA) to target folate receptors overexpressed in the cancer cells. This targeting vehicle provided remote-controlled delivery of this high toxic cargo cocktail at the tumor site, ensuring extra specificity that can avoid acute toxicity, where release of Dox and Pt(IV) was achieved upon NIR 808 nm diode laser irradiation. The dehybridization set the Dox free to bind the cell nucleus and cellular reductants reduced Pt(IV) to yield toxic Pt(II), becoming an active drug. The in vitro and in vivo studies revealed that this external stimulus responsive combination drug delivery was significantly effective.

Original languageEnglish
Pages (from-to)4382-4393
Number of pages12
JournalACS Applied Materials and Interfaces
Volume6
Issue number6
DOIs
Publication statusPublished - 2014 Mar 26

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Chemotherapy
Oligonucleotides
Prodrugs
Ablation
Nanorods
Drug delivery
Doxorubicin
Cisplatin
DNA
Poisons
Folic Acid
Complementary DNA
Cells
Acids
Drug Carriers
Single-Stranded DNA
Reducing Agents
Laser beam effects
Intercalation
Platinum

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

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title = "Oligonucleotides-assembled au nanorod-assisted cancer photothermal ablation and combination chemotherapy with targeted dual-drug delivery of doxorubicin and cisplatin prodrug",
abstract = "External stimuli responsive dual drugs carrier was synthesized with Au nanorods (NRs) as the platform. On Au NRs, single stranded DNAs were assembled using 5′ thiol end. Following this, complementary DNA (cDNA) strands were hybridized. This hybridized double stranded DNA facilitated doxorubicin (Dox) intercalation into the duplexes. The cDNA designed with the 5′ amine functional group assisted to tether platinum [Pt(IV)] prodrugs by establishing amide bond with the acid group at the axial ligand. The other axial acid group in Pt(IV) prodrugs was conjugated with the folic acid (FA) to target folate receptors overexpressed in the cancer cells. This targeting vehicle provided remote-controlled delivery of this high toxic cargo cocktail at the tumor site, ensuring extra specificity that can avoid acute toxicity, where release of Dox and Pt(IV) was achieved upon NIR 808 nm diode laser irradiation. The dehybridization set the Dox free to bind the cell nucleus and cellular reductants reduced Pt(IV) to yield toxic Pt(II), becoming an active drug. The in vitro and in vivo studies revealed that this external stimulus responsive combination drug delivery was significantly effective.",
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Oligonucleotides-assembled au nanorod-assisted cancer photothermal ablation and combination chemotherapy with targeted dual-drug delivery of doxorubicin and cisplatin prodrug. / Shanmugam, Vijayakumar; Chien, Yi Hsin; Cheng, Yu Sheng; Liu, Tzu Yu; Huang, Chih Chia; Su, Chia Hao; Chen, Yu Sen; Kumar, Umesh; Hsu, Hua Fen; Yeh, Chen Sheng.

In: ACS Applied Materials and Interfaces, Vol. 6, No. 6, 26.03.2014, p. 4382-4393.

Research output: Contribution to journalArticle

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T1 - Oligonucleotides-assembled au nanorod-assisted cancer photothermal ablation and combination chemotherapy with targeted dual-drug delivery of doxorubicin and cisplatin prodrug

AU - Shanmugam, Vijayakumar

AU - Chien, Yi Hsin

AU - Cheng, Yu Sheng

AU - Liu, Tzu Yu

AU - Huang, Chih Chia

AU - Su, Chia Hao

AU - Chen, Yu Sen

AU - Kumar, Umesh

AU - Hsu, Hua Fen

AU - Yeh, Chen Sheng

PY - 2014/3/26

Y1 - 2014/3/26

N2 - External stimuli responsive dual drugs carrier was synthesized with Au nanorods (NRs) as the platform. On Au NRs, single stranded DNAs were assembled using 5′ thiol end. Following this, complementary DNA (cDNA) strands were hybridized. This hybridized double stranded DNA facilitated doxorubicin (Dox) intercalation into the duplexes. The cDNA designed with the 5′ amine functional group assisted to tether platinum [Pt(IV)] prodrugs by establishing amide bond with the acid group at the axial ligand. The other axial acid group in Pt(IV) prodrugs was conjugated with the folic acid (FA) to target folate receptors overexpressed in the cancer cells. This targeting vehicle provided remote-controlled delivery of this high toxic cargo cocktail at the tumor site, ensuring extra specificity that can avoid acute toxicity, where release of Dox and Pt(IV) was achieved upon NIR 808 nm diode laser irradiation. The dehybridization set the Dox free to bind the cell nucleus and cellular reductants reduced Pt(IV) to yield toxic Pt(II), becoming an active drug. The in vitro and in vivo studies revealed that this external stimulus responsive combination drug delivery was significantly effective.

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