On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood

Lien Yu Hung, Wei Chun Tsai, Chien Yu Fu, Nai Jung Chiang, Yu Chun Wang, Yan-Shen Shan, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages563-564
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16-10-0916-10-13

Fingerprint

Biomarkers
Liver
Blood
Microfluidics

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Hung, L. Y., Tsai, W. C., Fu, C. Y., Chiang, N. J., Wang, Y. C., Shan, Y-S., & Lee, G. B. (2016). On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 563-564). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Hung, Lien Yu ; Tsai, Wei Chun ; Fu, Chien Yu ; Chiang, Nai Jung ; Wang, Yu Chun ; Shan, Yan-Shen ; Lee, Gwo Bin. / On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 563-564 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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title = "On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood",
abstract = "Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.",
author = "Hung, {Lien Yu} and Tsai, {Wei Chun} and Fu, {Chien Yu} and Chiang, {Nai Jung} and Wang, {Yu Chun} and Yan-Shen Shan and Lee, {Gwo Bin}",
year = "2016",
month = "1",
day = "1",
language = "English",
series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",
publisher = "Chemical and Biological Microsystems Society",
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}

Hung, LY, Tsai, WC, Fu, CY, Chiang, NJ, Wang, YC, Shan, Y-S & Lee, GB 2016, On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 563-564, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16-10-09.

On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. / Hung, Lien Yu; Tsai, Wei Chun; Fu, Chien Yu; Chiang, Nai Jung; Wang, Yu Chun; Shan, Yan-Shen; Lee, Gwo Bin.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 563-564 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood

AU - Hung, Lien Yu

AU - Tsai, Wei Chun

AU - Fu, Chien Yu

AU - Chiang, Nai Jung

AU - Wang, Yu Chun

AU - Shan, Yan-Shen

AU - Lee, Gwo Bin

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.

AB - Cholangiocarcinoma (CCA) is the second most common primary liver cancer. There are several existing biomarkers targeting liver cancer. However, none of them are specific to CCA. It is therefore challenging to diagnose CCA in the early stages. In this study, a new integrated microfluidic system was developed for rapid, on-chip cell capture and immunofluorescence (IF) staining with CCA specific biomarkers for CCA clinical diagnosis. Rare CCA cells were successfully captured and stained from clinical bile juice and whole blood.

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M3 - Conference contribution

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T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 563

EP - 564

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

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Hung LY, Tsai WC, Fu CY, Chiang NJ, Wang YC, Shan Y-S et al. On-chip cholangiocarcinoma cell capture and immunofluorescence staining for clinical diagnosis by using bile juice and whole blood. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 563-564. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).