On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

Yao Nan Wang, Jik Chang Leong, Chin Lung Chang, Chang Hsien Tai, Chien Hsiung Tsai, Lung-Ming Fu, Jr Ming Miao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

Original languageEnglish
Title of host publicationProceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Pages137-142
Number of pages6
DOIs
Publication statusPublished - 2010 Jul 12
EventASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 - Shanghai, China
Duration: 2009 Dec 182009 Dec 21

Publication series

NameProceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Volume1

Other

OtherASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
CountryChina
CityShanghai
Period09-12-1809-12-21

Fingerprint

Fluorescence
Fiber optics
Polydimethylsiloxane
Blood
Particle size
Cells
Geometry
Lasers
Experiments
baysilon

All Science Journal Classification (ASJC) codes

  • Fluid Flow and Transfer Processes

Cite this

Wang, Y. N., Leong, J. C., Chang, C. L., Tai, C. H., Tsai, C. H., Fu, L-M., & Miao, J. M. (2010). On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 (pp. 137-142). (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1). https://doi.org/10.1115/MNHMT2009-18298
Wang, Yao Nan ; Leong, Jik Chang ; Chang, Chin Lung ; Tai, Chang Hsien ; Tsai, Chien Hsiung ; Fu, Lung-Ming ; Miao, Jr Ming. / On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. pp. 137-142 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009).
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author = "Wang, {Yao Nan} and Leong, {Jik Chang} and Chang, {Chin Lung} and Tai, {Chang Hsien} and Tsai, {Chien Hsiung} and Lung-Ming Fu and Miao, {Jr Ming}",
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Wang, YN, Leong, JC, Chang, CL, Tai, CH, Tsai, CH, Fu, L-M & Miao, JM 2010, On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. in Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, vol. 1, pp. 137-142, ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, Shanghai, China, 09-12-18. https://doi.org/10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. / Wang, Yao Nan; Leong, Jik Chang; Chang, Chin Lung; Tai, Chang Hsien; Tsai, Chien Hsiung; Fu, Lung-Ming; Miao, Jr Ming.

Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 137-142 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Wang, Yao Nan

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AU - Tai, Chang Hsien

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AU - Fu, Lung-Ming

AU - Miao, Jr Ming

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Wang YN, Leong JC, Chang CL, Tai CH, Tsai CH, Fu L-M et al. On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 137-142. (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009). https://doi.org/10.1115/MNHMT2009-18298