On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

Yao Nan Wang; Jik Chang Leong; Chin Lung Chang; Chang Hsien Tai; Chien Hsiung Tsai; Lung Ming Fu; Jr Ming Miao

doi:10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

Yao Nan Wang
, Jik Chang Leong
, Chin Lung Chang
, Chang Hsien Tai
, Chien Hsiung Tsai
, Lung Ming Fu
, Jr Ming Miao

Department of Engineering Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

Original language	English
Title of host publication	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Pages	137-142
Number of pages	6
DOIs	https://doi.org/10.1115/MNHMT2009-18298
Publication status	Published - 2010 Jul 12
Event	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 - Shanghai, China Duration: 2009 Dec 18 → 2009 Dec 21

Publication series

Name	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Volume	1

Other

Other	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Country/Territory	China
City	Shanghai
Period	09-12-18 → 09-12-21

All Science Journal Classification (ASJC) codes

Fluid Flow and Transfer Processes

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10.1115/MNHMT2009-18298

Cite this

Wang, Y. N., Leong, J. C., Chang, C. L., Tai, C. H., Tsai, C. H., Fu, L. M., & Miao, J. M. (2010). On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 (pp. 137-142). (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1). https://doi.org/10.1115/MNHMT2009-18298

Wang, Yao Nan ; Leong, Jik Chang ; Chang, Chin Lung et al. / On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. pp. 137-142 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009).

@inproceedings{b5cd392f595741ac8a45e52b7afb0105,

title = "On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing",

abstract = "In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.",

author = "Wang, \{Yao Nan\} and Leong, \{Jik Chang\} and Chang, \{Chin Lung\} and Tai, \{Chang Hsien\} and Tsai, \{Chien Hsiung\} and Fu, \{Lung Ming\} and Miao, \{Jr Ming\}",

year = "2010",

month = jul,

day = "12",

doi = "10.1115/MNHMT2009-18298",

language = "English",

isbn = "9780791843895",

series = "Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009",

pages = "137--142",

booktitle = "Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009",

note = "ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 ; Conference date: 18-12-2009 Through 21-12-2009",

}

Wang, YN, Leong, JC, Chang, CL, Tai, CH, Tsai, CH, Fu, LM & Miao, JM 2010, On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. in Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, vol. 1, pp. 137-142, ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, Shanghai, China, 09-12-18. https://doi.org/10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. / Wang, Yao Nan; Leong, Jik Chang; Chang, Chin Lung et al.
Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 137-142 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

AU - Wang, Yao Nan

AU - Leong, Jik Chang

AU - Chang, Chin Lung

AU - Tai, Chang Hsien

AU - Tsai, Chien Hsiung

AU - Fu, Lung Ming

AU - Miao, Jr Ming

PY - 2010/7/12

Y1 - 2010/7/12

N2 - In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

AB - In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

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BT - Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009

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Wang YN, Leong JC, Chang CL, Tai CH, Tsai CH, Fu LM et al. On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 137-142. (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009). doi: 10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

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