On-demand dielectrophoretic immobilization and high-resolution imaging of C. elegans in microfluids

Liang Huang, Peng Zhao, Junhan Wu, Han Sheng Chuang, Wenhui Wang

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Caenorhabditis elegans (C. elegans) is a significant model organism in scientific research. However, considerable operations, such as neuron microcurgery and monitoring of development, require to silence the worm to achieve high-resolution imaging. To date, immobilization techniques being on-demand, low-injury, rapid, reversible, and easy to implement is highly desirable. This paper reports a dielectrophoretic method for on-demand immobilization of C. elegans in a microfluidic environment. Using thick electrodes and optimized parameters, we achieved high-efficiency and reversible immobilization of C. elegans of different stages, and demonstrated successful high-resolution imaging of immobilized nematode for important application. To the best of our knowledge, this is the first report showing on-demand, highly-efficient, and reversible immobilization of C. elegans for high-resolution imaging with uniform dielectrophoretic (DEP) forces generated from thick all polydimethylsiloxane (PDMS) electrodes. We believe this simple, efficient sensing and actuating lab-on-a-chip platform can largely facilitate the research of C. elegans biologists.

Original languageEnglish
Pages (from-to)703-708
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume259
DOIs
Publication statusPublished - 2018 Apr 15

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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