On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays

Shu Ping Lin, Themis R. Kyriakides, Jia-Jin Chen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Despite many successful applications of microelectrode arrays (MEAs), typical two-dimensional in-vitro cultures do not project the full scale of the cell growth environment in the three-dimensional (3D) in-vivo setting. This study aims to on-line monitor in-vitro cell growth in a 3D matrix on the surface-modified MEAs with a dynamic perfusion culture system. A 3D matrix consisting of poly(ethylene glycol) hydrogel supplemented with poly-d-lysine was subsequently synthesized in situ on the self-assembled monolayer modified MEAs. FTIR spectrum analysis revealed a peak at 2100 cm-1 due to the degradation of the structure of the 3D matrix. After 2 wks, microscopic examination revealed that the non-degraded area was around 1500 μm2 and provided enough space for cell growth. Fluorescence microscopy revealed that the degraded 3D matrix was non-cytotoxic allowing the growth of NIH3T3 fibroblasts and cortical neurons in vitro. Time-course changes of total impedance including resistance and reactance were recorded for 8 days to evaluate the cell growth in the 3D matrix on the MEA. A consistent trend reflecting changes of reactance and total impedance was observed. These in-vitro assays demonstrate that our 3D matrix can construct a biomimetic system for cell growth and analysis of cell surface interactions.

Original languageEnglish
Pages (from-to)3110-3117
Number of pages8
JournalBiomaterials
Volume30
Issue number17
DOIs
Publication statusPublished - 2009 Jun 1

Fingerprint

Microelectrodes
Cell growth
Observation
Growth
Electric Impedance
Hydrogel
Fluorescence microscopy
Biomimetics
Self assembled monolayers
Fibroblasts
Ethylene Glycol
Hydrogels
Spectrum analysis
Polyethylene glycols
Lysine
Neurons
Fourier Transform Infrared Spectroscopy
Assays
Microscopic examination
Fluorescence Microscopy

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

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abstract = "Despite many successful applications of microelectrode arrays (MEAs), typical two-dimensional in-vitro cultures do not project the full scale of the cell growth environment in the three-dimensional (3D) in-vivo setting. This study aims to on-line monitor in-vitro cell growth in a 3D matrix on the surface-modified MEAs with a dynamic perfusion culture system. A 3D matrix consisting of poly(ethylene glycol) hydrogel supplemented with poly-d-lysine was subsequently synthesized in situ on the self-assembled monolayer modified MEAs. FTIR spectrum analysis revealed a peak at 2100 cm-1 due to the degradation of the structure of the 3D matrix. After 2 wks, microscopic examination revealed that the non-degraded area was around 1500 μm2 and provided enough space for cell growth. Fluorescence microscopy revealed that the degraded 3D matrix was non-cytotoxic allowing the growth of NIH3T3 fibroblasts and cortical neurons in vitro. Time-course changes of total impedance including resistance and reactance were recorded for 8 days to evaluate the cell growth in the 3D matrix on the MEA. A consistent trend reflecting changes of reactance and total impedance was observed. These in-vitro assays demonstrate that our 3D matrix can construct a biomimetic system for cell growth and analysis of cell surface interactions.",
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On-line observation of cell growth in a three-dimensional matrix on surface-modified microelectrode arrays. / Lin, Shu Ping; Kyriakides, Themis R.; Chen, Jia-Jin.

In: Biomaterials, Vol. 30, No. 17, 01.06.2009, p. 3110-3117.

Research output: Contribution to journalArticle

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