On-line state estimation and control in glutamic acid production

An automatic computer control system for glutamic acid production has been developed. A method based on empirical reaction subspace and singular value decomposition was presented for on-line estimation of cell concentration, glutamic acid, and total sugar. The only on-line measured state variable was the oxygen uptake rate. The estimated cell concentration was used as an index for the addition of penicillin. The estimated total sugar concentration was used for system identification. Adaptive control was then applied to manipulate the substrate feeding rate. The total sugar concentration was maintained at a given value during the period of fed-batch culture for glutamic acid production.