TY - JOUR
T1 - One-dimensional modeling and simulations of migration of cultured fibroblasts
AU - Wu, Pei Jung
AU - Lin, Chou Ching K.
AU - Ju, Ming Shaung
N1 - Funding Information:
This research was supported by a grant from the National Council of Science Taiwan (98-2221-E-006-029-MY3). Drs. Chou-Ching K. Lin and Ming Shaung Ju contributed equally to this project.
PY - 2014/4
Y1 - 2014/4
N2 - Cell migration is crucial for many physiological functions such as wound healing, immuno-response and carcinogenesis. In this study an one-dimensional model of migration of fibroblasts was developed by modeling and integrating five subcellular processes, namely, actin protrusion, focal adhesion formation, stress fiber formation, polarization and retraction. The direction of migration was determined by polarization, which was related to direction of the stiffness gradient of the substrate. By controlling intensity of ultraviolet exposure on type-I collagen, a substrate with a stiffness gradient could be fabricated. Kinematic analyses of positions of the cell front, the nucleus and the cell rear, were utilized as inputs to the model. Simulation results of five live NIH 3T3 fibroblasts showed that the model was capable of simulating fast moving, slow moving and back-and-forth moving of the cells on the substrate.
AB - Cell migration is crucial for many physiological functions such as wound healing, immuno-response and carcinogenesis. In this study an one-dimensional model of migration of fibroblasts was developed by modeling and integrating five subcellular processes, namely, actin protrusion, focal adhesion formation, stress fiber formation, polarization and retraction. The direction of migration was determined by polarization, which was related to direction of the stiffness gradient of the substrate. By controlling intensity of ultraviolet exposure on type-I collagen, a substrate with a stiffness gradient could be fabricated. Kinematic analyses of positions of the cell front, the nucleus and the cell rear, were utilized as inputs to the model. Simulation results of five live NIH 3T3 fibroblasts showed that the model was capable of simulating fast moving, slow moving and back-and-forth moving of the cells on the substrate.
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U2 - 10.1142/S0219519414500274
DO - 10.1142/S0219519414500274
M3 - Article
AN - SCOPUS:84896797287
SN - 0219-5194
VL - 14
JO - Journal of Mechanics in Medicine and Biology
JF - Journal of Mechanics in Medicine and Biology
IS - 2
M1 - 1450027
ER -