One-step transformation of yeast in stationary phase

Dz Chi Chen, Bei Chang Yang, Tsong Teh Kuo

Research output: Contribution to journalArticlepeer-review

552 Citations (Scopus)

Abstract

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

Original languageEnglish
Pages (from-to)83-84
Number of pages2
JournalCurrent Genetics
Volume21
Issue number1
DOIs
Publication statusPublished - 1992 Jan 1

All Science Journal Classification (ASJC) codes

  • Genetics

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