One-step transformation of yeast in stationary phase

Dz Chi Chen, Bei-Chang Yang, Tsong Teh Kuo

Research output: Contribution to journalArticle

523 Citations (Scopus)

Abstract

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

Original languageEnglish
Pages (from-to)83-84
Number of pages2
JournalCurrent Genetics
Volume21
Issue number1
DOIs
Publication statusPublished - 1992 Jan 1

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Yeasts
DNA
Alkalies
Plasmids
Ions

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Chen, Dz Chi ; Yang, Bei-Chang ; Kuo, Tsong Teh. / One-step transformation of yeast in stationary phase. In: Current Genetics. 1992 ; Vol. 21, No. 1. pp. 83-84.
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One-step transformation of yeast in stationary phase. / Chen, Dz Chi; Yang, Bei-Chang; Kuo, Tsong Teh.

In: Current Genetics, Vol. 21, No. 1, 01.01.1992, p. 83-84.

Research output: Contribution to journalArticle

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