Optical detection of photo-activated protein using Brewster angle microscopy

Y. Li, S. Gorelik, N. Tomczak, H. Y. Song, M. J. Lear, J. Hobley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).

Original languageEnglish
Title of host publication2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
DOIs
Publication statusPublished - 2008
Event2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008 - Singapore, Singapore
Duration: 2008 Dec 82008 Dec 11

Publication series

Name2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008

Other

Other2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
CountrySingapore
CitySingapore
Period08-12-0808-12-11

All Science Journal Classification (ASJC) codes

  • Computer Networks and Communications
  • Electrical and Electronic Engineering

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