Optical detection of photo-activated protein using Brewster angle microscopy

Y. Li; S. Gorelik; N. Tomczak; H. Y. Song; M. J. Lear; J. Hobley

doi:10.1109/IPGC.2008.4781381

Optical detection of photo-activated protein using Brewster angle microscopy

Y. Li, S. Gorelik, N. Tomczak, H. Y. Song, M. J. Lear, J. Hobley

Department of Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).

Original language	English
Title of host publication	2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
DOIs	https://doi.org/10.1109/IPGC.2008.4781381
Publication status	Published - 2008
Event	2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008 - Singapore, Singapore Duration: 2008 Dec 8 → 2008 Dec 11

Publication series

Name	2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008

Other

Other	2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
Country/Territory	Singapore
City	Singapore
Period	08-12-08 → 08-12-11

All Science Journal Classification (ASJC) codes

Computer Networks and Communications
Electrical and Electronic Engineering

Access to Document

10.1109/IPGC.2008.4781381

Cite this

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title = "Optical detection of photo-activated protein using Brewster angle microscopy",

abstract = "Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).",

author = "Y. Li and S. Gorelik and N. Tomczak and Song, {H. Y.} and Lear, {M. J.} and J. Hobley",

year = "2008",

doi = "10.1109/IPGC.2008.4781381",

language = "English",

isbn = "9781424429059",

series = "2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008",

booktitle = "2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008",

}

Li, Y, Gorelik, S, Tomczak, N, Song, HY, Lear, MJ & Hobley, J 2008, Optical detection of photo-activated protein using Brewster angle microscopy. in 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008., 4781381, 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008, 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008, Singapore, Singapore, 08-12-08. https://doi.org/10.1109/IPGC.2008.4781381

TY - GEN

T1 - Optical detection of photo-activated protein using Brewster angle microscopy

AU - Li, Y.

AU - Gorelik, S.

AU - Tomczak, N.

AU - Song, H. Y.

AU - Lear, M. J.

AU - Hobley, J.

PY - 2008

Y1 - 2008

N2 - Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).

AB - Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).

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U2 - 10.1109/IPGC.2008.4781381

DO - 10.1109/IPGC.2008.4781381

M3 - Conference contribution

AN - SCOPUS:67649655851

SN - 9781424429059

T3 - 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008

BT - 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008

T2 - 2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008

Y2 - 8 December 2008 through 11 December 2008

ER -

Optical detection of photo-activated protein using Brewster angle microscopy

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