Optimization of an electroporation system for gene transfection by taguchi method

Yu Cheng Lin, Ching Yuan Hung, Keng Shiang Huang, Chun Sheng Fang, Chih Hui Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study demonstrates an optimized electroporation (EP) system for gene transfection by the Taguchi method. The parameters of the EP system, having an influence on the transfection rate, were optimized, resulting in optimized values of 50 μm electrode gap, 80 μg/mL pEGFP-N1 concentration, 6 V pulse voltage, and 2-pulse per time with above 95% confidence. The results show that the gene transfection efficiency is highly enhanced due to the dramatically increased pEGFP-N1 concentration by the electrostatic force. With optimized factors, the pEGFP-N1 plasmid was successfully transfected into the basal cell carcinoma cell line (BCC) with 35.89 % average transfection rate.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1425-1427
Number of pages3
ISBN (Print)0974361119, 9780974361116
Publication statusPublished - 2005 Jan 1
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: 2005 Oct 92005 Oct 13

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period05-10-0905-10-13

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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