Abstract
In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation [1], tissue sterilization [2], wound care [3], etc. However, the effect of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules to wound healing is still controversy, therefore diagnosis plasma parameters is necessary. In this work, non-thermal N2/Ar microplasma is first measured the average temperature, reactive oxygen species with different percentage of gas addition. Based on these databases, the optimization parameters were chosen to treatment fibroblast cells in vitro study and burn wound in vivo study. The primary results showed that 0.5% N2/Ar micro-plasma exposure at 17 W can particularly be used to enhance the cells proliferation and migration. The cells number increased one and half folds for plasma exposure time of 5 or 10 sec, followed by cell culture for 48 hrs. The cell coverage rate rose 20% for the same plasma exposure time, followed by cell culture for 6 or 12 hrs. Based on the results in vitro study, N2/Ar micro-plasma at 13 W was applied to improve healing on the second degree burn wound mice through the wound area reduction, followed by repairing tissue assessments.
| Original language | English |
|---|---|
| Title of host publication | BME-HUST 2016 - 3rd International Conference on Biomedical Engineering |
| Publisher | Institute of Electrical and Electronics Engineers Inc. |
| Pages | 146-149 |
| Number of pages | 4 |
| ISBN (Electronic) | 9781509010974 |
| DOIs | |
| Publication status | Published - 2016 Dec 12 |
| Event | 3rd International Conference on Biomedical Engineering, BME-HUST 2016 - Hanoi, Viet Nam Duration: 2016 Oct 5 → 2016 Oct 6 |
Publication series
| Name | BME-HUST 2016 - 3rd International Conference on Biomedical Engineering |
|---|
Conference
| Conference | 3rd International Conference on Biomedical Engineering, BME-HUST 2016 |
|---|---|
| Country | Viet Nam |
| City | Hanoi |
| Period | 16-10-05 → 16-10-06 |
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All Science Journal Classification (ASJC) codes
- Biomedical Engineering
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Optimization of micro-plasma parameters for wound healing. / Hien, Ngo Thi Minh; Linh, Huynh Quang; Liao, Jiunn-Der.
BME-HUST 2016 - 3rd International Conference on Biomedical Engineering. Institute of Electrical and Electronics Engineers Inc., 2016. p. 146-149 7782095 (BME-HUST 2016 - 3rd International Conference on Biomedical Engineering).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Optimization of micro-plasma parameters for wound healing
AU - Hien, Ngo Thi Minh
AU - Linh, Huynh Quang
AU - Liao, Jiunn-Der
PY - 2016/12/12
Y1 - 2016/12/12
N2 - In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation [1], tissue sterilization [2], wound care [3], etc. However, the effect of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules to wound healing is still controversy, therefore diagnosis plasma parameters is necessary. In this work, non-thermal N2/Ar microplasma is first measured the average temperature, reactive oxygen species with different percentage of gas addition. Based on these databases, the optimization parameters were chosen to treatment fibroblast cells in vitro study and burn wound in vivo study. The primary results showed that 0.5% N2/Ar micro-plasma exposure at 17 W can particularly be used to enhance the cells proliferation and migration. The cells number increased one and half folds for plasma exposure time of 5 or 10 sec, followed by cell culture for 48 hrs. The cell coverage rate rose 20% for the same plasma exposure time, followed by cell culture for 6 or 12 hrs. Based on the results in vitro study, N2/Ar micro-plasma at 13 W was applied to improve healing on the second degree burn wound mice through the wound area reduction, followed by repairing tissue assessments.
AB - In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation [1], tissue sterilization [2], wound care [3], etc. However, the effect of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules to wound healing is still controversy, therefore diagnosis plasma parameters is necessary. In this work, non-thermal N2/Ar microplasma is first measured the average temperature, reactive oxygen species with different percentage of gas addition. Based on these databases, the optimization parameters were chosen to treatment fibroblast cells in vitro study and burn wound in vivo study. The primary results showed that 0.5% N2/Ar micro-plasma exposure at 17 W can particularly be used to enhance the cells proliferation and migration. The cells number increased one and half folds for plasma exposure time of 5 or 10 sec, followed by cell culture for 48 hrs. The cell coverage rate rose 20% for the same plasma exposure time, followed by cell culture for 6 or 12 hrs. Based on the results in vitro study, N2/Ar micro-plasma at 13 W was applied to improve healing on the second degree burn wound mice through the wound area reduction, followed by repairing tissue assessments.
UR - http://www.scopus.com/inward/record.url?scp=85010433864&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010433864&partnerID=8YFLogxK
U2 - 10.1109/BME-HUST.2016.7782095
DO - 10.1109/BME-HUST.2016.7782095
M3 - Conference contribution
T3 - BME-HUST 2016 - 3rd International Conference on Biomedical Engineering
SP - 146
EP - 149
BT - BME-HUST 2016 - 3rd International Conference on Biomedical Engineering
PB - Institute of Electrical and Electronics Engineers Inc.
ER -