Optimization of micro-plasma parameters for wound healing

Ngo Thi Minh Hien, Huynh Quang Linh, Jiunn-Der Liao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation [1], tissue sterilization [2], wound care [3], etc. However, the effect of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules to wound healing is still controversy, therefore diagnosis plasma parameters is necessary. In this work, non-thermal N2/Ar microplasma is first measured the average temperature, reactive oxygen species with different percentage of gas addition. Based on these databases, the optimization parameters were chosen to treatment fibroblast cells in vitro study and burn wound in vivo study. The primary results showed that 0.5% N2/Ar micro-plasma exposure at 17 W can particularly be used to enhance the cells proliferation and migration. The cells number increased one and half folds for plasma exposure time of 5 or 10 sec, followed by cell culture for 48 hrs. The cell coverage rate rose 20% for the same plasma exposure time, followed by cell culture for 6 or 12 hrs. Based on the results in vitro study, N2/Ar micro-plasma at 13 W was applied to improve healing on the second degree burn wound mice through the wound area reduction, followed by repairing tissue assessments.

Original languageEnglish
Title of host publicationBME-HUST 2016 - 3rd International Conference on Biomedical Engineering
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages146-149
Number of pages4
ISBN (Electronic)9781509010974
DOIs
Publication statusPublished - 2016 Dec 12
Event3rd International Conference on Biomedical Engineering, BME-HUST 2016 - Hanoi, Viet Nam
Duration: 2016 Oct 52016 Oct 6

Publication series

NameBME-HUST 2016 - 3rd International Conference on Biomedical Engineering

Conference

Conference3rd International Conference on Biomedical Engineering, BME-HUST 2016
CountryViet Nam
CityHanoi
Period16-10-0516-10-06

Fingerprint

Plasmas
Cell culture
Tissue
Cell proliferation
Fibroblasts
Coagulation
Gases
Excited states
Blood
Cells
Molecules
Oxygen
Temperature

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

Hien, N. T. M., Linh, H. Q., & Liao, J-D. (2016). Optimization of micro-plasma parameters for wound healing. In BME-HUST 2016 - 3rd International Conference on Biomedical Engineering (pp. 146-149). [7782095] (BME-HUST 2016 - 3rd International Conference on Biomedical Engineering). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/BME-HUST.2016.7782095
Hien, Ngo Thi Minh ; Linh, Huynh Quang ; Liao, Jiunn-Der. / Optimization of micro-plasma parameters for wound healing. BME-HUST 2016 - 3rd International Conference on Biomedical Engineering. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 146-149 (BME-HUST 2016 - 3rd International Conference on Biomedical Engineering).
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abstract = "In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation [1], tissue sterilization [2], wound care [3], etc. However, the effect of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules to wound healing is still controversy, therefore diagnosis plasma parameters is necessary. In this work, non-thermal N2/Ar microplasma is first measured the average temperature, reactive oxygen species with different percentage of gas addition. Based on these databases, the optimization parameters were chosen to treatment fibroblast cells in vitro study and burn wound in vivo study. The primary results showed that 0.5{\%} N2/Ar micro-plasma exposure at 17 W can particularly be used to enhance the cells proliferation and migration. The cells number increased one and half folds for plasma exposure time of 5 or 10 sec, followed by cell culture for 48 hrs. The cell coverage rate rose 20{\%} for the same plasma exposure time, followed by cell culture for 6 or 12 hrs. Based on the results in vitro study, N2/Ar micro-plasma at 13 W was applied to improve healing on the second degree burn wound mice through the wound area reduction, followed by repairing tissue assessments.",
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Hien, NTM, Linh, HQ & Liao, J-D 2016, Optimization of micro-plasma parameters for wound healing. in BME-HUST 2016 - 3rd International Conference on Biomedical Engineering., 7782095, BME-HUST 2016 - 3rd International Conference on Biomedical Engineering, Institute of Electrical and Electronics Engineers Inc., pp. 146-149, 3rd International Conference on Biomedical Engineering, BME-HUST 2016, Hanoi, Viet Nam, 16-10-05. https://doi.org/10.1109/BME-HUST.2016.7782095

Optimization of micro-plasma parameters for wound healing. / Hien, Ngo Thi Minh; Linh, Huynh Quang; Liao, Jiunn-Der.

BME-HUST 2016 - 3rd International Conference on Biomedical Engineering. Institute of Electrical and Electronics Engineers Inc., 2016. p. 146-149 7782095 (BME-HUST 2016 - 3rd International Conference on Biomedical Engineering).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Hien NTM, Linh HQ, Liao J-D. Optimization of micro-plasma parameters for wound healing. In BME-HUST 2016 - 3rd International Conference on Biomedical Engineering. Institute of Electrical and Electronics Engineers Inc. 2016. p. 146-149. 7782095. (BME-HUST 2016 - 3rd International Conference on Biomedical Engineering). https://doi.org/10.1109/BME-HUST.2016.7782095