In recent years, atmospheric micro-plasma has various applications in the biomedical field, including blood coagulation , tissue sterilization , wound care , etc. However, the effect of atmospheric micro-plasma, mainly through particle excited state, and some of the gas molecules to wound healing is still controversy, therefore diagnosis plasma parameters is necessary. In this work, non-thermal N2/Ar microplasma is first measured the average temperature, reactive oxygen species with different percentage of gas addition. Based on these databases, the optimization parameters were chosen to treatment fibroblast cells in vitro study and burn wound in vivo study. The primary results showed that 0.5% N2/Ar micro-plasma exposure at 17 W can particularly be used to enhance the cells proliferation and migration. The cells number increased one and half folds for plasma exposure time of 5 or 10 sec, followed by cell culture for 48 hrs. The cell coverage rate rose 20% for the same plasma exposure time, followed by cell culture for 6 or 12 hrs. Based on the results in vitro study, N2/Ar micro-plasma at 13 W was applied to improve healing on the second degree burn wound mice through the wound area reduction, followed by repairing tissue assessments.