Optimized method for determining free L-Cysteine in rat plasma by high-performance liquid chromatography with the 4-aminosulfonyl-7-fluoro-2,1,3- benzoxadiazole conversion reagent

Lila Otani, Shinya Ogawa, Zhilei Zhao, Kyoko Nakazawa, Shunsuke Umehara, Etsuro Yoshimura, Sue-Joan Chang, Hisanori Kato

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5 Citations (Scopus)

Abstract

The analytical method was optimized for L-cysteine (Cys) in rat plasma with co-existing L-cystine (Cyss). We observed that more than 100% Cyss in rat plasma was converted to Cys under typical conditions for the conversion with 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). Another conversion reagent, 4-aminosulfonyl- 7-fluoro-2,1,3-benzoxadiazole (ABD-F), was then employed, with which the reaction could be carried out at a low temperature without the use of a reducing reagent. Under the optimized conditions of 4°C and pH 8.3, the conversion ratio of Cyss to Cys in rat plasma was as low as 5-7%. We determined the Cys concentration in plasma of the portal vein of rats that had been orally administered with Cys and Cyss by applying this method. The result indicated that Cys administration and also Cyss administration effectively increased the plasma Cys level. The method developed in this study is well suited for determining the thiol compounds in biological samples.

Original languageEnglish
Pages (from-to)2119-2124
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number11
DOIs
Publication statusPublished - 2011 Nov 30

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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