TY - JOUR
T1 - Optimizing lipase production from isolated Burkholderia sp.
AU - Liu, Chien Hung
AU - Huang, Chien Chang
AU - Wang, Yao Wen
AU - Chang, Jo Shu
N1 - Funding Information:
This study was financially supported by the Research Grant NSC100-3113-E-006-016-, NSC100-2218-E-126-002-, and NSC99-2221-E-006-137-MY3 from Taiwan's National Science Council. The support from the “Top University Grant” of National Cheng Kung University is also appreciated.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - Lipase produced from microorganisms can be used in many industrial applications, such as detergent formulation, oil/fat degradation, pharmaceutical synthesis, biodiesel and cosmetics production. Due to the increase in demand for lipase, increasing attention has been paid to how to produce it efficiently and economically. In this work, lipase production from an isolated Burkholderia sp. C20 was enhanced by using the response surface methodology (RSM) to optimize the medium composition and the fermentation condition. The titer of lipase production was 16.7. U/ml under the optimal conditions with yeast extract of 2.175. g/l and olive oil of 5.54. ml/l. The lipase production was improved to 22.6. U/ml in a fermentor with an aeration rate of 1. vvm and a controlled pH at 6.0. The Burkholderia lipase exhibits good thermal and pH stabilities for 21. h incubation and the zymography analysis showed that the molecular weight of the lipase is about 23. kDa.
AB - Lipase produced from microorganisms can be used in many industrial applications, such as detergent formulation, oil/fat degradation, pharmaceutical synthesis, biodiesel and cosmetics production. Due to the increase in demand for lipase, increasing attention has been paid to how to produce it efficiently and economically. In this work, lipase production from an isolated Burkholderia sp. C20 was enhanced by using the response surface methodology (RSM) to optimize the medium composition and the fermentation condition. The titer of lipase production was 16.7. U/ml under the optimal conditions with yeast extract of 2.175. g/l and olive oil of 5.54. ml/l. The lipase production was improved to 22.6. U/ml in a fermentor with an aeration rate of 1. vvm and a controlled pH at 6.0. The Burkholderia lipase exhibits good thermal and pH stabilities for 21. h incubation and the zymography analysis showed that the molecular weight of the lipase is about 23. kDa.
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U2 - 10.1016/j.jtice.2012.02.004
DO - 10.1016/j.jtice.2012.02.004
M3 - Article
AN - SCOPUS:84862794764
SN - 1876-1070
VL - 43
SP - 511
EP - 516
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
IS - 4
ER -