Optoelectrokinetically-enabled diagnosis of diabetic retinopathy with dual biomarkers lipocalin 1 and tumor necrosis factor-α

Han Sheng Chuang, Hsiao Neng Lin, Jen Yi Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Diabetic retinopathy (DR) is an ocular morbidity that usually occurs in the retina of patients with chronic diabetes. Without proper medical intervention, DR eventually may lead to blindness. To meet the clinical need, this study developed an immunosensing technique based on optoelectrokinetically enabled beads for simultaneously detecting dual biomarkers, lipocalin 1 (LCN1) and tumor necrosis factor-α (TNF-α). A sandwiched configuration comprising capture antibody–antigen-probe antibody was formed by conjugating particles with capture antibodies. The additional dye-labeled secondary antibodies were eventually attached to the probe antibodies to determine the presence of the target biomarkers. The concentration of the target biomarkers was measured based on fluorescence intensity. The optoelectrokinetic technique, named as rapid electrokinetic patterning (REP), was used to enhance the fluorescence signal by concentrating the functionalized particles into a cluster. REP exhibited limit of detection as low as 110 pg/mL and was operated with simultaneous applications of optical illumination and electric field. Particles of different sizes were labeled with different dye colors to detect the corresponding DR biomarkers. The efficiency and accuracy of the proposed technique were increased by cross-matching multiple biomarkers in the tear of patients with DR. The optoelectrokinetic system can be used for treatments performed by ophthalmologists and can be extended to diagnose other oculopathies in the future.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages978-981
Number of pages4
ISBN (Electronic)9781510897571
Publication statusPublished - 2018 Jan 1
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume2

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan
CityKaohsiung
Period18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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