Optoelectrokinetically-Enabled Signal Enhancement for a Bead-Based FRET Fluorescence Immunoassay

Han Sheng Chuang, Kuan Chih Wang, Dar Bin Hsieh, Yong Qing Fu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bead-based immunoassay has been growing as a promising technology in the point-of-care diagnostics due to great flexibility. For samples with dilute analytes, the colloidal particles can concentrate dispersed analytes and act as carriers for advanced particle manipulation. To realize a rapid in-situ immunoassay, Firster resonance energy transfer (FRET) was used herein to ensure only the target antigen lipocalin 1 (LCN1) to be detected. The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. In addition, an optoelectrokinetic technique was used to further concentrate the functionalized particles to enhance the FRET fluorescent signal. The detection was usually completed within several tens of seconds. A comparison between the based-based diagnostics coupled with the optoelectrokinetic technique and a conventional ELISA reader showed nearly 100-fold improvement of the bio-signal was made. The technique is expected to bring hopes for diagnosing diseases with dilute biomarkers in the early stage.

Original languageEnglish
Title of host publication2014 International Symposium on Optomechatronic Technologies, ISOT 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages101-104
Number of pages4
ISBN (Electronic)9781467367523
DOIs
Publication statusPublished - 2014 Jan 1
Event2014 International Symposium on Optomechatronic Technologies, ISOT 2014 - Seattle, United States
Duration: 2014 Nov 52014 Nov 7

Publication series

Name2014 International Symposium on Optomechatronic Technologies, ISOT 2014

Other

Other2014 International Symposium on Optomechatronic Technologies, ISOT 2014
CountryUnited States
CitySeattle
Period14-11-0514-11-07

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

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