Optogenetic deconstructing the mechanism of neuroplasticity modulated by repetitive cortical stimulation

Chun Wei Wu, Cho Han Hsieh, Jia Jin Jason Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cortical Theta burst stimulation (TBS) may modulate dopamine-dependent corticostriatal plasticity via long-term potentiation/depression (LTP/LTD) -like mechanism. This gives TBS a therapeutic potential for Parkinson’s disease (PD) and other neural disorder. To unravel the mechanism beneath TBS-induced neuroplasticity, a novel optogenetic simulating method will be applied. CaMKII promoter-driven channelrhodopsin-2 (ChR2) will be delivered into glutamatergic pyramidal neuron in motor cortex by lentivirus. Under emitting of precisely controlled blue laser guided by fiber optics, the excitatory circuit in layer V will be elicited with TBS paradigm. Cortical excitabilities were examined by measuring optogenetic simulation-evoked potential and motor-evoked potential (MEP). In summary, we proposed a novel optical-TBS rodent model, which revealed the function role of glutamatergic circuit in motor plasticity.

Original languageEnglish
Title of host publication1st Global Conference on Biomedical Engineering and 9th Asian-Pacific Conference on Medical and Biological Engineering
EditorsFong-Chin Su, Ming-Long Yeh, Shyh-Hau Wang
PublisherSpringer Verlag
Pages260-263
Number of pages4
ISBN (Electronic)9783319122618
Publication statusPublished - 2015 Jan 1
Event1st Global Conference on Biomedical Engineering, GCBME 2014 and 9th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2014 - Tainan, Taiwan
Duration: 2014 Oct 92014 Oct 12

Publication series

NameIFMBE Proceedings
Volume47
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Other

Other1st Global Conference on Biomedical Engineering, GCBME 2014 and 9th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2014
CountryTaiwan
CityTainan
Period14-10-0914-10-12

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

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  • Cite this

    Wu, C. W., Hsieh, C. H., & Chen, J. J. J. (2015). Optogenetic deconstructing the mechanism of neuroplasticity modulated by repetitive cortical stimulation. In F-C. Su, M-L. Yeh, & S-H. Wang (Eds.), 1st Global Conference on Biomedical Engineering and 9th Asian-Pacific Conference on Medical and Biological Engineering (pp. 260-263). (IFMBE Proceedings; Vol. 47). Springer Verlag.