Orientation control of zebrafish embryos using artificial cilia in a 3D flow-through microchannel

Karthick Mani, Tsung Chun Chang Chien, Chia Yuan Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The zebrafish has emerged as an important vertebrate model for genetic screens and new drug development due to its significant characteristics such as optical transparency, rapid ex vivo growth, and high genetic similarity to humans. Despite these benefits, the scale of zebrafish studies is still limited as a result of the lack of a robust method to manipulate zebrafish during screening. In this work a new microfluidic channel layout in conjunction with a series of magnetically actuated artificial cilia were employed to provide orientation control of zebrafish larvae with axial rotation capability. This method enables 0-15 degrees of rotation inside the microchannel with high accuracy and less detrimental impact, as opposed to the conventional methods. In addition, the bioactivity of tested larvas remains stable with no significant difference to those in the control group during the time-lapse imaging. The presented platform along with the provided analytical paradigm is forecasted to be beneficial to facilitate the zebrafish screening using microfluidics in pharmaceutical industry.

Original languageEnglish
Title of host publication2015 IEEE 10th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages15-19
Number of pages5
ISBN (Electronic)9781467366953
DOIs
Publication statusPublished - 2015 Jul 1
Event10th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015 - Xi'an, China
Duration: 2015 Apr 72015 Apr 11

Publication series

Name2015 IEEE 10th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015

Other

Other10th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015
CountryChina
CityXi'an
Period15-04-0715-04-11

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Hardware and Architecture

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