Osteochondral tissue regeneration using a tyramine-modified bilayered plga scaffold combined with articular chondrocytes in a porcine model

Tzu Hsiang Lin, Hsueh Chun Wang, Wen Hui Cheng, Horng Chaung Hsu, Ming-Long Yeh

Research output: Contribution to journalArticle

Abstract

Repairing damaged articular cartilage is challenging due to the limited regenerative capacity of hyaline cartilage. In this study, we fabricated a bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold with small (200–300 µm) and large (200–500 µm) pores by salt leaching to stimulate chondrocyte differentiation, cartilage formation, and endochondral ossification. The scaffold surface was treated with tyramine to promote scaffold integration into native tissue. Porcine chondrocytes retained a round shape during differentiation when grown on the small pore size scaffold, and had a fibroblast-like morphology during transdifferentiation in the large pore size scaffold after five days of culture. Tyramine-treated scaffolds with mixed pore sizes seeded with chondrocytes were pressed into three-mm porcine osteochondral defects; tyramine treatment enhanced the adhesion of the small pore size scaffold to osteochondral tissue and increased glycosaminoglycan and collagen type II (Col II) contents, while reducing collagen type X (Col X) production in the cartilage layer. Col X content was higher for scaffolds with a large pore size, which was accompanied by the enhanced generation of subchondral bone. Thus, chondrocytes seeded in tyramine-treated bilayered scaffolds with small and large pores in the upper and lower parts, respectively, can promote osteochondral regeneration and integration for articular cartilage repair.

Original languageEnglish
Article number326
JournalInternational journal of molecular sciences
Volume20
Issue number2
DOIs
Publication statusPublished - 2019 Jan 2

Fingerprint

Tissue regeneration
Tyramine
Chondrocytes
regeneration
Scaffolds
cartilage
Regeneration
Swine
Cartilage
Joints
Collagen Type X
porosity
Pore size
Articular Cartilage
collagens
Collagen
Hyaline Cartilage
Scaffolds (biology)
Collagen Type II
Glycosaminoglycans

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

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title = "Osteochondral tissue regeneration using a tyramine-modified bilayered plga scaffold combined with articular chondrocytes in a porcine model",
abstract = "Repairing damaged articular cartilage is challenging due to the limited regenerative capacity of hyaline cartilage. In this study, we fabricated a bilayered poly (lactic-co-glycolic acid) (PLGA) scaffold with small (200–300 µm) and large (200–500 µm) pores by salt leaching to stimulate chondrocyte differentiation, cartilage formation, and endochondral ossification. The scaffold surface was treated with tyramine to promote scaffold integration into native tissue. Porcine chondrocytes retained a round shape during differentiation when grown on the small pore size scaffold, and had a fibroblast-like morphology during transdifferentiation in the large pore size scaffold after five days of culture. Tyramine-treated scaffolds with mixed pore sizes seeded with chondrocytes were pressed into three-mm porcine osteochondral defects; tyramine treatment enhanced the adhesion of the small pore size scaffold to osteochondral tissue and increased glycosaminoglycan and collagen type II (Col II) contents, while reducing collagen type X (Col X) production in the cartilage layer. Col X content was higher for scaffolds with a large pore size, which was accompanied by the enhanced generation of subchondral bone. Thus, chondrocytes seeded in tyramine-treated bilayered scaffolds with small and large pores in the upper and lower parts, respectively, can promote osteochondral regeneration and integration for articular cartilage repair.",
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Osteochondral tissue regeneration using a tyramine-modified bilayered plga scaffold combined with articular chondrocytes in a porcine model. / Lin, Tzu Hsiang; Wang, Hsueh Chun; Cheng, Wen Hui; Hsu, Horng Chaung; Yeh, Ming-Long.

In: International journal of molecular sciences, Vol. 20, No. 2, 326, 02.01.2019.

Research output: Contribution to journalArticle

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