Overexpression of a single membrane component from the Bacillus mer operon enhanced mercury resistance in an Escherichia coli host

Ju Liang Hsieh, Ching Yi Chen, Jo Shu Chang, Ginro Endo, Chieh Chen Huang

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Overexpression of a mercuric ion binding protein, MerP, from the mercury resistance operon genes of Gram-positive bacterial strain Bacillus megaterium MB1 and from Gram-negative bacterial strain Pseudomonas aeruginosa K-62 was found to enhance the mercury resistance level of Escherichia coli host cells, even though they share only 27.3% identity. Immunoblot analysis showed that MerP (BMerP) from Bacillus could be expressed on the membrane fraction of E. coli cells. Treated with 10 μM Hg2+, a recombinant strain harboring the BMerP gene significantly improved, showing a 27% increase in mercuric ion adsorption capacity, 16% better than that of a Pseudomonas merP gene (PMerP)-harboring strain. While multiple heavy metals co-existed, the mercuric ion adsorption capacity of the BMerP-harboring E. coli was not affected while that of the PMerP-harboring strain decreased. These results suggest that BMerP can act as a bio-adsorbent compartmentalizing the toxic mercuric ion on the cell membrane and enhancing resistance.

Original languageEnglish
Pages (from-to)1494-1499
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number6
DOIs
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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