Overexpression of MDM‐2 mRNA and mutation of the p53 tumor suppressor gene in bladder carcinoma cell lines

Ya‐Tse ‐T Cheng, Yu‐Ling ‐L Li, Jeng Dau Wu, Shih‐Bin ‐B Long, Tzong‐Shin ‐S Tzai, Ching‐Cherng ‐C Tzeng, Ming-Derg Lai

Research output: Contribution to journalArticle

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Abstract

To investigate the importance of oncogenes and tumor suppressor genes in bladder carcinogenesis, we determined the status of the expression of the MDM‐2 and p53 genes and genetic alterations in the p53 gene in five bladder carcinoma cell lines and one kidney urothelial carcinoma cell line. Overexpression of MDM‐2 mRNA was observed in three bladder carcinoma cell lines, J82, SCaBER, and BFTC‐905. Amplification of the MDM‐2 gene was not detected in any of the six cell lines by Southern analysis. The deletion in the p53 gene was observed in J82, and point mutation was detected in J82 and BFTC‐909, the kidney urothelial carcinoma cell line. In contrast, no mutations were found in codons 12, 13, and 61 in the Ha‐ras and Ki‐ras genes in these six cell lines. These results indicate that alterations in the p53‐regulated pathway are important in bladder carcinogenesis. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalMolecular Carcinogenesis
Volume13
Issue number3
DOIs
Publication statusPublished - 1995 Jan 1

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Tumor Suppressor Genes
Urinary Bladder
Carcinoma
Cell Line
Messenger RNA
Mutation
p53 Genes
Carcinogenesis
Kidney
Gene Amplification
Oncogenes
Point Mutation
Codon
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cancer Research

Cite this

Cheng, Ya‐Tse ‐T ; Li, Yu‐Ling ‐L ; Wu, Jeng Dau ; Long, Shih‐Bin ‐B ; Tzai, Tzong‐Shin ‐S ; Tzeng, Ching‐Cherng ‐C ; Lai, Ming-Derg. / Overexpression of MDM‐2 mRNA and mutation of the p53 tumor suppressor gene in bladder carcinoma cell lines. In: Molecular Carcinogenesis. 1995 ; Vol. 13, No. 3. pp. 173-181.
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Overexpression of MDM‐2 mRNA and mutation of the p53 tumor suppressor gene in bladder carcinoma cell lines. / Cheng, Ya‐Tse ‐T; Li, Yu‐Ling ‐L; Wu, Jeng Dau; Long, Shih‐Bin ‐B; Tzai, Tzong‐Shin ‐S; Tzeng, Ching‐Cherng ‐C; Lai, Ming-Derg.

In: Molecular Carcinogenesis, Vol. 13, No. 3, 01.01.1995, p. 173-181.

Research output: Contribution to journalArticle

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AU - Li, Yu‐Ling ‐L

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AU - Long, Shih‐Bin ‐B

AU - Tzai, Tzong‐Shin ‐S

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N2 - To investigate the importance of oncogenes and tumor suppressor genes in bladder carcinogenesis, we determined the status of the expression of the MDM‐2 and p53 genes and genetic alterations in the p53 gene in five bladder carcinoma cell lines and one kidney urothelial carcinoma cell line. Overexpression of MDM‐2 mRNA was observed in three bladder carcinoma cell lines, J82, SCaBER, and BFTC‐905. Amplification of the MDM‐2 gene was not detected in any of the six cell lines by Southern analysis. The deletion in the p53 gene was observed in J82, and point mutation was detected in J82 and BFTC‐909, the kidney urothelial carcinoma cell line. In contrast, no mutations were found in codons 12, 13, and 61 in the Ha‐ras and Ki‐ras genes in these six cell lines. These results indicate that alterations in the p53‐regulated pathway are important in bladder carcinogenesis. © 1995 Wiley‐Liss, Inc.

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