Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome

Yumi Iwadate, Hirofumi Honda, Haruhiko Sato, Masayuki Hashimoto, Jun Ichi Kato

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The construction of engineered bacterial cells with a reduced genome allows the investigation of molecular mechanisms that may be cryptic in wild-type strains and derivatives. Previously, a large-scale combined deletion mutant of Escherichia coli that lacked 29.7% of the parental chromosome was constructed by combining large chromosome deletions. In this work, we improved the system for making markerless-chromosomal deletions and obtained mutants with a genome that lacked up to 38.9% of the parental chromosome. Although the large-scale deletion mutants possessed genes needed for resistance to oxidative stress, including superoxide dismutase, catalase, and RpoS, they were sensitive to menadione, which induces reactive oxygen species during stationary phase. Small genome size did not necessarily correlate with greater sensitivity to menadione as several mutants with large deletions were more resistant to menadione. The sensitivity to menadione depended on whether the mutants were grown aerobically or anaerobically, suggesting that the mechanism governing menadione resistance depended on the oxygen tension of the growth medium. Further analysis of the large-scale deletion mutants should help identify the regulatory networks that are important for cellular defense against oxidative stress.

Original languageEnglish
Pages (from-to)25-33
Number of pages9
JournalFEMS Microbiology Letters
Volume322
Issue number1
DOIs
Publication statusPublished - 2011 Sep 1

Fingerprint

Vitamin K 3
Oxidative Stress
Genome
Escherichia coli
Chromosomes
Genome Size
Chromosome Deletion
Catalase
Superoxide Dismutase
Reactive Oxygen Species
Oxygen
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Iwadate, Yumi ; Honda, Hirofumi ; Sato, Haruhiko ; Hashimoto, Masayuki ; Kato, Jun Ichi. / Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome. In: FEMS Microbiology Letters. 2011 ; Vol. 322, No. 1. pp. 25-33.
@article{322a3ab8ee96442c9a94b524c1b57de9,
title = "Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome",
abstract = "The construction of engineered bacterial cells with a reduced genome allows the investigation of molecular mechanisms that may be cryptic in wild-type strains and derivatives. Previously, a large-scale combined deletion mutant of Escherichia coli that lacked 29.7{\%} of the parental chromosome was constructed by combining large chromosome deletions. In this work, we improved the system for making markerless-chromosomal deletions and obtained mutants with a genome that lacked up to 38.9{\%} of the parental chromosome. Although the large-scale deletion mutants possessed genes needed for resistance to oxidative stress, including superoxide dismutase, catalase, and RpoS, they were sensitive to menadione, which induces reactive oxygen species during stationary phase. Small genome size did not necessarily correlate with greater sensitivity to menadione as several mutants with large deletions were more resistant to menadione. The sensitivity to menadione depended on whether the mutants were grown aerobically or anaerobically, suggesting that the mechanism governing menadione resistance depended on the oxygen tension of the growth medium. Further analysis of the large-scale deletion mutants should help identify the regulatory networks that are important for cellular defense against oxidative stress.",
author = "Yumi Iwadate and Hirofumi Honda and Haruhiko Sato and Masayuki Hashimoto and Kato, {Jun Ichi}",
year = "2011",
month = "9",
day = "1",
doi = "10.1111/j.1574-6968.2011.02331.x",
language = "English",
volume = "322",
pages = "25--33",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome. / Iwadate, Yumi; Honda, Hirofumi; Sato, Haruhiko; Hashimoto, Masayuki; Kato, Jun Ichi.

In: FEMS Microbiology Letters, Vol. 322, No. 1, 01.09.2011, p. 25-33.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Oxidative stress sensitivity of engineered Escherichia coli cells with a reduced genome

AU - Iwadate, Yumi

AU - Honda, Hirofumi

AU - Sato, Haruhiko

AU - Hashimoto, Masayuki

AU - Kato, Jun Ichi

PY - 2011/9/1

Y1 - 2011/9/1

N2 - The construction of engineered bacterial cells with a reduced genome allows the investigation of molecular mechanisms that may be cryptic in wild-type strains and derivatives. Previously, a large-scale combined deletion mutant of Escherichia coli that lacked 29.7% of the parental chromosome was constructed by combining large chromosome deletions. In this work, we improved the system for making markerless-chromosomal deletions and obtained mutants with a genome that lacked up to 38.9% of the parental chromosome. Although the large-scale deletion mutants possessed genes needed for resistance to oxidative stress, including superoxide dismutase, catalase, and RpoS, they were sensitive to menadione, which induces reactive oxygen species during stationary phase. Small genome size did not necessarily correlate with greater sensitivity to menadione as several mutants with large deletions were more resistant to menadione. The sensitivity to menadione depended on whether the mutants were grown aerobically or anaerobically, suggesting that the mechanism governing menadione resistance depended on the oxygen tension of the growth medium. Further analysis of the large-scale deletion mutants should help identify the regulatory networks that are important for cellular defense against oxidative stress.

AB - The construction of engineered bacterial cells with a reduced genome allows the investigation of molecular mechanisms that may be cryptic in wild-type strains and derivatives. Previously, a large-scale combined deletion mutant of Escherichia coli that lacked 29.7% of the parental chromosome was constructed by combining large chromosome deletions. In this work, we improved the system for making markerless-chromosomal deletions and obtained mutants with a genome that lacked up to 38.9% of the parental chromosome. Although the large-scale deletion mutants possessed genes needed for resistance to oxidative stress, including superoxide dismutase, catalase, and RpoS, they were sensitive to menadione, which induces reactive oxygen species during stationary phase. Small genome size did not necessarily correlate with greater sensitivity to menadione as several mutants with large deletions were more resistant to menadione. The sensitivity to menadione depended on whether the mutants were grown aerobically or anaerobically, suggesting that the mechanism governing menadione resistance depended on the oxygen tension of the growth medium. Further analysis of the large-scale deletion mutants should help identify the regulatory networks that are important for cellular defense against oxidative stress.

UR - http://www.scopus.com/inward/record.url?scp=79960645969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960645969&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2011.02331.x

DO - 10.1111/j.1574-6968.2011.02331.x

M3 - Article

VL - 322

SP - 25

EP - 33

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -