Abstract
Degradation of extracellular matrix by hyaluronidase increases murine L929 cell sensitivity to tumor necrosis factor (TNF) cytotoxicity. Seeding and culturing L929 cells onto the matrix of serum fetuin and the hyaluronate-binding inter-α-inhibitor resulted in inhibition of hyaluronidase-enhanced TNF killing, suggesting that the release of these proteins from hyaluronidase-degraded matrix confers cellular TNF susceptibility. Metabolic labeling studies showed that hyaluronidase mediated de novo protein synthesis and downregulated several proteins in L929 cells. Specifically, hyaluronidase upregulated p53 protein expression (>200%) but downregulated a p85 inter-α-inhibitor-like protein (>90%) in L929 cells, whereas it had no effect on the protein levels of ICH-1, Bcl-xL, Bcl-2, Fas ligand, CAS (cellular apoptosis susceptible protein), TIAR (an RNA-binding protein) and α-tubulin. Conceivably, hyaluronidase enhancement of TNF sensitivity in L929 cells is p53-dependent and the matrix inter-α-inhibitor contributes a protective role against TNF cytotoxicity. Copyright (C) 1998 Elsevier Science Ireland Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 45-54 |
| Number of pages | 10 |
| Journal | Cancer Letters |
| Volume | 131 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1998 Sep 11 |
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All Science Journal Classification (ASJC) codes
- Cancer Research
- Molecular Biology
- Oncology
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p53 overexpression and downregulation of inter-α-inhibitor are associated with hyaluronidase enhancement of TNF cytotoxicity in L929 fibroblasts. / Chang, Nan-Shan; Carey, Gregory; Pratt, Nicole; Chu, Elaina; Ou, Melody.
In: Cancer Letters, Vol. 131, No. 1, 11.09.1998, p. 45-54.Research output: Contribution to journal › Article
TY - JOUR
T1 - p53 overexpression and downregulation of inter-α-inhibitor are associated with hyaluronidase enhancement of TNF cytotoxicity in L929 fibroblasts
AU - Chang, Nan-Shan
AU - Carey, Gregory
AU - Pratt, Nicole
AU - Chu, Elaina
AU - Ou, Melody
PY - 1998/9/11
Y1 - 1998/9/11
N2 - Degradation of extracellular matrix by hyaluronidase increases murine L929 cell sensitivity to tumor necrosis factor (TNF) cytotoxicity. Seeding and culturing L929 cells onto the matrix of serum fetuin and the hyaluronate-binding inter-α-inhibitor resulted in inhibition of hyaluronidase-enhanced TNF killing, suggesting that the release of these proteins from hyaluronidase-degraded matrix confers cellular TNF susceptibility. Metabolic labeling studies showed that hyaluronidase mediated de novo protein synthesis and downregulated several proteins in L929 cells. Specifically, hyaluronidase upregulated p53 protein expression (>200%) but downregulated a p85 inter-α-inhibitor-like protein (>90%) in L929 cells, whereas it had no effect on the protein levels of ICH-1, Bcl-xL, Bcl-2, Fas ligand, CAS (cellular apoptosis susceptible protein), TIAR (an RNA-binding protein) and α-tubulin. Conceivably, hyaluronidase enhancement of TNF sensitivity in L929 cells is p53-dependent and the matrix inter-α-inhibitor contributes a protective role against TNF cytotoxicity. Copyright (C) 1998 Elsevier Science Ireland Ltd.
AB - Degradation of extracellular matrix by hyaluronidase increases murine L929 cell sensitivity to tumor necrosis factor (TNF) cytotoxicity. Seeding and culturing L929 cells onto the matrix of serum fetuin and the hyaluronate-binding inter-α-inhibitor resulted in inhibition of hyaluronidase-enhanced TNF killing, suggesting that the release of these proteins from hyaluronidase-degraded matrix confers cellular TNF susceptibility. Metabolic labeling studies showed that hyaluronidase mediated de novo protein synthesis and downregulated several proteins in L929 cells. Specifically, hyaluronidase upregulated p53 protein expression (>200%) but downregulated a p85 inter-α-inhibitor-like protein (>90%) in L929 cells, whereas it had no effect on the protein levels of ICH-1, Bcl-xL, Bcl-2, Fas ligand, CAS (cellular apoptosis susceptible protein), TIAR (an RNA-binding protein) and α-tubulin. Conceivably, hyaluronidase enhancement of TNF sensitivity in L929 cells is p53-dependent and the matrix inter-α-inhibitor contributes a protective role against TNF cytotoxicity. Copyright (C) 1998 Elsevier Science Ireland Ltd.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032508577&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(98)00200-6
DO - 10.1016/S0304-3835(98)00200-6
M3 - Article
VL - 131
SP - 45
EP - 54
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -