Paclitaxel-loaded stabilizer-free poly(D,L-lactide-co-glycolide) nanoparticles conjugated with quantum dots for reversion of anti-cancer drug resistance and cancer cellular imaging

Wen Shuo Kuo, Yu Cian Ku, Hei Tin Sei, Fong Yu Cheng, Chen-Sheng Yeh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.

Original languageEnglish
Pages (from-to)923-934
Number of pages12
JournalJournal of the Chinese Chemical Society
Volume56
Issue number5
DOIs
Publication statusPublished - 2009 Jan 1

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Paclitaxel
Semiconductor quantum dots
Nanoparticles
Imaging techniques
Pharmaceutical Preparations
Cells
Cytotoxicity
polylactic acid-polyglycolic acid copolymer
Drug Carriers
P-Glycoprotein
Cell membranes
Verapamil
Curing

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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title = "Paclitaxel-loaded stabilizer-free poly(D,L-lactide-co-glycolide) nanoparticles conjugated with quantum dots for reversion of anti-cancer drug resistance and cancer cellular imaging",
abstract = "We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.",
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Paclitaxel-loaded stabilizer-free poly(D,L-lactide-co-glycolide) nanoparticles conjugated with quantum dots for reversion of anti-cancer drug resistance and cancer cellular imaging. / Kuo, Wen Shuo; Ku, Yu Cian; Sei, Hei Tin; Cheng, Fong Yu; Yeh, Chen-Sheng.

In: Journal of the Chinese Chemical Society, Vol. 56, No. 5, 01.01.2009, p. 923-934.

Research output: Contribution to journalArticle

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