Abstract
We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.
| Original language | English |
|---|---|
| Pages (from-to) | 923-934 |
| Number of pages | 12 |
| Journal | Journal of the Chinese Chemical Society |
| Volume | 56 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Paclitaxel-loaded stabilizer-free poly(D,L-lactide-co-glycolide) nanoparticles conjugated with quantum dots for reversion of anti-cancer drug resistance and cancer cellular imaging. / Kuo, Wen Shuo; Ku, Yu Cian; Sei, Hei Tin; Cheng, Fong Yu; Yeh, Chen-Sheng.
In: Journal of the Chinese Chemical Society, Vol. 56, No. 5, 01.01.2009, p. 923-934.Research output: Contribution to journal › Article
TY - JOUR
T1 - Paclitaxel-loaded stabilizer-free poly(D,L-lactide-co-glycolide) nanoparticles conjugated with quantum dots for reversion of anti-cancer drug resistance and cancer cellular imaging
AU - Kuo, Wen Shuo
AU - Ku, Yu Cian
AU - Sei, Hei Tin
AU - Cheng, Fong Yu
AU - Yeh, Chen-Sheng
PY - 2009/1/1
Y1 - 2009/1/1
N2 - We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.
AB - We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.
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U2 - 10.1002/jccs.200900136
DO - 10.1002/jccs.200900136
M3 - Article
VL - 56
SP - 923
EP - 934
JO - Journal of the Chinese Chemical Society
JF - Journal of the Chinese Chemical Society
SN - 0009-4536
IS - 5
ER -