Partial reversion of transformed phenotype of B104 cancer cells by antisense nucleic acids

Yueh Tsern Shaw, Shu Huey Chang, Shean Tai Chiou, Wen Chang Chang, Ming Derg Lai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We used antisense RNA to inhibit the expression of oncogene neu and investigated the effects of diminished neu expression on the phenotypes of B104 cells containing activated oncogene neu. Antisense MT-neu and pSV-neo plasmids were cotransfected into neuroblastoma B104 cells. Southern analysis showed the integration of antineu DNA into B104 cells. The expression of neu was inhibited up to 90% as quantitated by immunoprecipitation. The growth rate and the potential to differentiate in these transfectants were not affected as compared to the parental cell lines. The ability to grow in soft agar was inhibited more than 90% in these transfectants. Our results indicated that antisense-RNA against a specific oncogene can decrease the tumorigenicity of tumor cells but may not be able to revert it to normal cells completely.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalCancer Letters
Volume69
Issue number1
DOIs
Publication statusPublished - 1993 Apr 15

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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