Participation of p97Eps8 in Src-mediated Transformation

Tzeng Horng Leu, Hsu Hua Yeh, Ching Chung Huang, Ya Chun Chuang, Shu Li Su, Ming Chei Maa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Histone acetylase and histone deacetylase are two crucial enzymes that determine the structure of chromatin, regulating gene expression. In this study, we observed that trichostatin A (TSA), a specific histone deacetylase inhibitor, could effectively inhibit the growth of v-Src-transformed (IV5) cells and abrogate their ability to form colonies in soft agar. Further analysis demonstrated that, although TSA reduced the expression of Eps8 in a dose- and time-dependent manner, both the protein expression and kinase activity of v-Src remained constant, and the abundance and phosphotyrosine levels of Src substrates, including cortactin, focal adhesion kinase, p130 Cas, paxillin, and Shc, were not altered. Notably, removal of TSA from the medium restored not only the expression of Eps8, but also cellular growth. Northern and reverse transcription-PCR analyses revealed the significant reduction of eps8 transcripts in TSA-treated IV5 cells relative to control cells. When active Src-expressing chicken embryonic cells were forced to overexpress p97Eps8, they became resistant to TSA-mediated anti-proliferation. Furthermore, using small interference RNA of eps8, we demonstrated the requirement for Eps8 in IV5 cell proliferation. Thus, our results highlight a critical role for p97Eps8 in TSA-exerted growth inhibition of v-Src-transformed cells.

Original languageEnglish
Pages (from-to)9875-9881
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number11
DOIs
Publication statusPublished - 2004 Mar 12

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Participation of p97<sup>Eps8</sup> in Src-mediated Transformation'. Together they form a unique fingerprint.

  • Cite this

    Leu, T. H., Yeh, H. H., Huang, C. C., Chuang, Y. C., Su, S. L., & Maa, M. C. (2004). Participation of p97Eps8 in Src-mediated Transformation. Journal of Biological Chemistry, 279(11), 9875-9881. https://doi.org/10.1074/jbc.M309884200