Patch clamping on plane glass - Fabrication of hourglass aperture and high-yield ion channel recording

Chang Yu Chen, Ting Yuan Tu, Chang Hung Chen, De Shien Jong, Andrew M. Wo

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Planar patch-clamp has revolutionized ion-channel measurement by eliminating laborious manipulation from the traditional micropipette approach and enabling high throughput. However, low yield in gigaseal formation and/or relatively high cost due to microfabricated processes are two main drawbacks. This paper presents patch clamping on glass substrate - an economical solution without sacrificing gigaseal yield rate. Two-stage CO2 laser drilling methodology was used to generate an hourglass, funnel-like aperture of a specified diameter with smooth and debris-free surfaces on 150 m borosilicate cover glass. For 1-3 m apertures as patch-clamp chips, seal resistance was tested on human embryonic kidney, Chinese hamster ovary, and Jurkat T lymphoma cells with a gigaseal success rate of 62.5%, 43.6% and 66.7% respectively. Results also demonstrated both whole-cell and single channel recording on endogenously expressed ion channels to confirm the capability of different patch configurations.

Original languageEnglish
Pages (from-to)2370-2380
Number of pages11
JournalLab on a Chip
Volume9
Issue number16
DOIs
Publication statusPublished - 2009 Jan 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Patch clamping on plane glass - Fabrication of hourglass aperture and high-yield ion channel recording'. Together they form a unique fingerprint.

  • Cite this