TY - GEN
T1 - Pathogen detection from phalaenopsis orchids by using an integrated microfluidic system
AU - Chang, Wen Hsin
AU - Yang, Sung Yi
AU - Wang, Chih Hung
AU - Lee, Gwo Bin
AU - Chen, Tzong-Yueh
AU - Li, Ping Chen
AU - Jan, Fuh Jyh
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Early detection of pathogens in high-value agricultural species is crucial. Therefore., many methods which can detect agricultural pathogens to prevent economic loss have been developed. Among them., immunoassays., nucleic acid hybridization and polymerase chain reaction have been demonstrated to detect pathogens in Phalaenopsis orchid successfully with satisfactory sensitivity and specificity. However., above-mentioned methods all have some disadvantages including lengthy process or requiring specialized laboratory facilities and well-trained technicians. The current study therefore presents an integrated micro fluidic system for rapid and automatic detection of pathogens in agricultural species. The entire procedure., including pathogen-specific ribonucleic acid (RNA) purification., nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection., can be automatically performed on a single chip within 65 minutes. Moreover., the detection module can be interchanged between a fluorescent detector and a turbidity detector., depending on the need of the operator. Furthermore., the developed system can detect pathogens directly from fresh agriculture tissues such as leaves and flowers of the Phalaneopsis orchids. This is the first time that an interchangeable integrated micro fluidic system for the detection of Phalaneopsis orchids has been demonstrated. Some of the most prevalent Phalaenopsis orchid pathogens., such as Cymbidium mosaic virus (CymMV) and Tomato spotted wilt virus (TSWV) for Phalaneopsis orchids were used in the current study to demonstrate the capabilities of the developed system. It is concluded that this system with dual detection units can directly detect pathogens from crude agricultural materials successfully.
AB - Early detection of pathogens in high-value agricultural species is crucial. Therefore., many methods which can detect agricultural pathogens to prevent economic loss have been developed. Among them., immunoassays., nucleic acid hybridization and polymerase chain reaction have been demonstrated to detect pathogens in Phalaenopsis orchid successfully with satisfactory sensitivity and specificity. However., above-mentioned methods all have some disadvantages including lengthy process or requiring specialized laboratory facilities and well-trained technicians. The current study therefore presents an integrated micro fluidic system for rapid and automatic detection of pathogens in agricultural species. The entire procedure., including pathogen-specific ribonucleic acid (RNA) purification., nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection., can be automatically performed on a single chip within 65 minutes. Moreover., the detection module can be interchanged between a fluorescent detector and a turbidity detector., depending on the need of the operator. Furthermore., the developed system can detect pathogens directly from fresh agriculture tissues such as leaves and flowers of the Phalaneopsis orchids. This is the first time that an interchangeable integrated micro fluidic system for the detection of Phalaneopsis orchids has been demonstrated. Some of the most prevalent Phalaenopsis orchid pathogens., such as Cymbidium mosaic virus (CymMV) and Tomato spotted wilt virus (TSWV) for Phalaneopsis orchids were used in the current study to demonstrate the capabilities of the developed system. It is concluded that this system with dual detection units can directly detect pathogens from crude agricultural materials successfully.
UR - http://www.scopus.com/inward/record.url?scp=84880217443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880217443&partnerID=8YFLogxK
U2 - 10.1109/NANOMED.2012.6509132
DO - 10.1109/NANOMED.2012.6509132
M3 - Conference contribution
AN - SCOPUS:84880217443
SN - 9781467351027
T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
SP - 6
EP - 10
BT - NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
T2 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
Y2 - 4 November 2012 through 7 November 2012
ER -