Pea chloroplast DnaJ-J8 and Toc12 are encoded by the same gene and localized in the stroma

Chi-Chou Chiu, Lih Jen Chen, Hsou Min Li

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Toc12 is a novel J domain-containing protein identified in pea (Pisum sativum) chloroplasts. It was shown to be an integral outer membrane protein localizing in the intermembrane space of the chloroplast envelope. Furthermore, Toc12 was shown to associate with an intermembrane space Hsp70, suggesting that Toc12 is important for protein translocation across the chloroplast envelope. Toc12 shares a high degree of sequence similarity with Arabidopsis (Arabidopsis thaliana) DnaJ-J8, which has been suggested to be a soluble protein of the chloroplast stroma. Here, we isolated genes encoding DnaJ-J8 from pea and found that Toc12 is a truncated clone of one of the pea DnaJ-J8s. Protein import analyses indicate that Toc12 and DnaJ-J8s possess a cleavable transit peptide and are localized in the stroma. Arabidopsis mutants with T-DNA insertions in the DnaJ-J8 gene show no defect in chloroplast protein import. Implications of these results in the energetics and mechanisms of chloroplast protein import are discussed.

Original languageEnglish
Pages (from-to)1172-1182
Number of pages11
JournalPlant Physiology
Volume154
Issue number3
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Peas
Chloroplasts
Chloroplast Proteins
peas
chloroplasts
Arabidopsis
imports
Genes
genes
Protein Transport
proteins
pea protein
Membrane Proteins
protein transport
Clone Cells
outer membrane proteins
Pisum sativum
Peptides
N-(8-aminooctyl)-5-iodonaphthalene-1-sulfonamide
Arabidopsis thaliana

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Chiu, Chi-Chou ; Chen, Lih Jen ; Li, Hsou Min. / Pea chloroplast DnaJ-J8 and Toc12 are encoded by the same gene and localized in the stroma. In: Plant Physiology. 2010 ; Vol. 154, No. 3. pp. 1172-1182.
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Pea chloroplast DnaJ-J8 and Toc12 are encoded by the same gene and localized in the stroma. / Chiu, Chi-Chou; Chen, Lih Jen; Li, Hsou Min.

In: Plant Physiology, Vol. 154, No. 3, 01.11.2010, p. 1172-1182.

Research output: Contribution to journalArticle

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